■D抗原变体被分类为弱,局部,极弱(DEL),可以使用分子测试进行区分。在智利,当地血液中心的实验室无法识别D抗原的变体,将它们转交给智利公共卫生研究所的参考实验室进行研究。所以,我们的目的是讨论在血清学分类中具有不同结果的样品中D抗原变体的分子分析结果.
■在Rh系统的D抗原分类中,将479份血清学结果不一致的样品送去进行分子分析。Rh表型用单克隆抗C,反C,反E,和通过直接凝集的抗e抗血清。为了找到D抗原,研究人员使用单克隆抗血清直接凝集和柱(凝胶)凝集法间接抗球蛋白测试。用序列特异性引物的聚合酶链反应(SSP-PCR)和测序进行分子分析。
■在332个样本(69.3%)中证实了D抗原变体的存在,血清学分类有初步差异。在这组差异样本中,弱RhD变异的频率为66%(219/332),极弱的RHD为28%(93/332),部分RhD为6%(20/332)。2型弱变异(27.4%),类型3(8.4%),48型(8.4%),1型(8.1%)是仅次于RHD*DEL43(28%)的下一个最普遍的变异。ccEe(R2r)表型是最频繁检测的(38.4%),并且存在于87%的RHD*DEL43样品中。E抗原与该变体的存在相关。我们的分析首次描述了智利的D抗原变体。最常见的变体是DEL型(RHD*DEL43)和弱(弱类型2),与ccDEe(R2r)表型相关。这些发现使我们能够表征智利的D抗原变体,根据获得的数据,为捐助者的管理设计策略,病人,和孕妇。
The D antigen variants are classified as weak, partial, and extremely weak (DEL) and can be differentiated using molecular tests. In Chile, the laboratories of local blood centers do not identify variants of the D antigen, referring them for study to the Reference Laboratory of the Public Health Institute of Chile. So, our aim was to talk about the results of the molecular analysis of variants of the D antigen in samples that had different results in the serological classification.
In the D antigen classification of the Rh system, 479 samples with serological discrepant results were sent for molecular analysis. The Rh phenotype was performed with monoclonal anti-C, anti-c, anti-E, and anti-e antisera by direct agglutination. To find the D antigen, researchers used direct agglutination with monoclonal antisera and indirect antiglobulin testing with the column (gel) agglutination method. Molecular analysis was performed with a polymerase chain reaction with sequence-specific primers (SSP-PCR) and sequencing.
The presence of D antigen variants was confirmed in 332 samples (69.3%), with an initial discrepancy in serological classification. In this group of discrepant samples, the frequency of weak RhD variants was 66% (219/332), that of extremely weak RhD was 28% (93/332), and that of partial RhD was 6% (20/332). The weak variants type 2 (27.4%), type 3 (8.4%), type 48 (8.4%), and type 1 (8.1%) were the next most prevalent variants after RHD*DEL43 (28%). The ccEe (R2r) phenotype was the most frequently detected (38.4%) and is present in 87% of the RHD*DEL43 samples. The E antigen is associated with the presence of this variant. Our analyses give the first description of D antigen variants in Chile. The most common variants are DEL type (RHD*DEL43) and weak (weak type 2), which are linked to the ccDEe (R2r) phenotype. These findings allow us to characterize the variants of the D antigen in Chile and, according to the obtained data, to design strategies for the management of donors, patients, and pregnant women.