■过敏性哮喘是一种呈现多种内型的疾病,目前没有治疗方法可以治愈,但可以缓解疾病症状。饮食干预在调节免疫反应方面越来越重要。此外,短链脂肪酸(SFCA),作为膳食纤维的主要产品由肠道细菌发酵,改善包括哮喘在内的不同疾病的发病机制和疾病负担。然而,肠和肺之间的连接和串扰知之甚少。
■在这项工作中,研究了高纤维饮食在基线和呼吸道病毒引起的疾病加重后对过敏性哮喘发展的作用.
■特此,对哮喘和非哮喘学前儿童气道疾病症状前后血清SCFA进行分析。此外,分析了高纤维饮食在屋尘螨提取物(HDM)诱导的过敏性哮喘的小鼠模型中以及在离体感染鼻病毒的离体肺和脾细胞中的作用。
■在这项研究中,与基线访视时哮喘患儿和对照组患儿相比,观察到有症状发作后哮喘患儿血清中SCFA3-羟丁酸的下降.在实验性哮喘中,在喂食高纤维饮食的小鼠中,减少肺GATA3+Th2型介导的炎症,观察到嗜酸性粒细胞中的粘液产生和胶原蛋白沉积以及Fcε受体Ia(FcεRIa)的表达。相比之下,在饲喂高纤维饮食的哮喘小鼠的肺中诱导CD8记忆效应T细胞。然后,用标准食物或富含纤维食物喂养的这些哮喘小鼠的总肺细胞被离体RV感染。这里,发现与来自标准食物喂养的哮喘小鼠的肺细胞相比,来自富含纤维的食物喂养的小鼠的肺细胞中的RVlbmRNA显着降低。寻找机制,来自富含纤维的哮喘小鼠的RV感染脾细胞中CD8+T细胞的增加,被观察到。
■有症状发作的恢复期学龄前哮喘儿童在基线就诊时与对照组和哮喘儿童相比,血清β-羟丁酸较少。富含纤维的饮食通过减少2型和IgE介导的免疫应答并在过敏性哮喘的鼠模型中诱导CD8+记忆效应T细胞而与抗炎作用以及抗过敏作用相关。最后,与饲喂标准食物的小鼠相比,饲喂富含纤维食物的哮喘小鼠的总肺细胞的鼻病毒(RV)离体感染导致RV负荷降低。此外,来自富含纤维的食物的哮喘小鼠的脾细胞在用RV离体感染后诱导的CD8T细胞。
■增加果胶等天然纤维含量的饮食干预将改善哮喘恶化。此外,哮喘患者呼吸道感染下调肠道SCFA,导致哮喘加重.
UNASSIGNED: Allergic Asthma is a disease presenting various endotypes and no current therapies act curative but alleviate disease symptoms. Dietary interventions are gaining increasing importance in regulating immune responses. Furthermore, short chain fatty acids (SFCA), as the main products of dietary fiber\'s fermentation by the gut bacteria, ameliorate the pathogenesis and disease burden of different illnesses including asthma. Nevertheless, the connection and crosstalk between the gut and lung is poorly understood.
UNASSIGNED: In this work, the role of high fiber diet on the development of allergic asthma at baseline and after exacerbation of disease induced by respiratory viruses was investigated.
UNASSIGNED: Hereby, SCFA in serum of asthmatic and non-asthmatic pre-school children before and after airway disease symptoms were analyzed. Moreover, the effect of high fiber diet in vivo in a murine model of house dust mite extract (HDM) induced allergic asthma and in the end in isolated lung and spleen cells infected ex vivo with Rhinovirus was analyzed.
UNASSIGNED: In this study, a decrease of the SCFA 3-Hydroxybutyric acid in serum of asthmatic children after symptomatic episodes at convalescent visit as compared to asthmatic and control children at baseline visit was observed. In experimental asthma, in mice fed with high fiber diet, a reduced lung
GATA3 + Th2 type mediated inflammation, mucus production and collagen deposition and expression of Fc epsilon receptor Ia (FcεRIa) in eosinophils was observed. By contrast, the CD8+ memory effector T cells were induced in the lungs of asthmatic mice fed with high fiber diet. Then, total lung cells from these asthmatic mice fed with either standard food or with fiber rich food were infected with RV ex vivo. Here, RV1b mRNA was found significantly reduced in the lung cells derived from fiber rich food fed mice as compared to those derived from standard food fed asthmatic mice. Looking for the mechanism, an increase in CD8+ T cells in RV infected spleen cells derived from fiber rich fed asthmatic mice, was observed.
UNASSIGNED: Convalescent preschool asthmatic children after a symptomatic episode have less serum ß-Hydroxybutyric acid as compared to control and asthmatic children at baseline visit. Fiber rich diet associated with anti-inflammatory effects as well as anti-allergic effects by decreasing Type 2 and IgE mediated immune responses and inducing CD8+ memory effector T cells in a murine model of allergic asthma. Finally, ex vivo infection with Rhinovirus (RV) of total lung cells from asthmatic mice fed with fiber rich food led to a decreased RV load as compared to mice fed with standard food. Moreover, spleen cells derived from asthmatic mice fed with fiber rich food induced CD8+ T cells after ex vivo infection with RV.
UNASSIGNED: Dietary interventions with increased content in natural fibers like pectins would ameliorate asthma exacerbations. Moreover, respiratory infection in asthma downregulated SCFA in the gut contributing to asthma exacerbations.