背景:肠道微生物和年龄都是影响疾病发展的因素。肠道微生物的群落结构受年龄的影响。
目的:绘制45岁以上个体随时间变化的肠道微生物谱图,探讨年龄与肠道微生物的相关性。
方法:从510名45岁以上的健康个体收集粪便样本。香农指数,辛普森指数,Ace指数,等。用于分析肠道微生物的多样性。β多样性分析,包括非度量多维缩放(NMDS),用于分析社区分布。采用线性判别分析(LDA)和随机森林(RF)算法对肠道微生物差异进行分析。使用趋势分析来绘制不同年龄的特征肠道微生物的丰度。
结果:45-49岁的个体具有最高的肠道细菌丰富度。15种特征肠道微生物,包括硅藻科和短双歧杆菌,通过射频算法进行筛选。65岁以上的个体中,肺炎杆菌和微病毒科的丰度较高。此外,布劳提亚的丰富,随着年龄的增长,Lubbockvirus和肠球菌梭状芽孢杆菌的数量减少,并且随着年龄的增长,克雷伯氏菌和普雷沃氏菌的数量增加。功能基因,如人类疾病和衰老,不同年龄个体之间存在显著差异。
结论:不同年龄的个体具有特征性的肠道微生物。肠道微生物群落结构的变化可能与年龄诱发的疾病有关。
Gut microbes and age are both factors that influence the development of disease. The community structure of gut microbes is affected by age.
To plot time-dependent gut microbe profiles in individuals over 45 years old and explore the correlation between age and gut microbes.
Fecal samples were collected from 510 healthy individuals over 45 years old. Shannon index, Simpson index, Ace index, etc. were used to analyze the diversity of gut microbes. The beta diversity analysis, including non-metric multidimensional scaling (NMDS), was used to analyze community distribution. Linear discriminant analysis (LDA) and random forest (RF) algorithm were used to analyze the differences of gut microbes. Trend analysis was used to plot the abundances of characteristic gut microbes in different ages.
The individuals aged 45-49 had the highest richness of gut bacteria. Fifteen characteristic gut microbes, including Siphoviridae and Bifidobacterium breve, were screened by RF algorithm. The abundance of Ligiactobacillus and Microviridae were higher in individuals older than 65 years. Moreover, the abundance of Blautia_A massiliensis, Lubbockvirus and Enterocloster clostridioformis decreased with age and the abundance of Klebsiella variicola and Prevotella increased with age. The functional genes, such as human diseases and aging, were significantly different among different aged individuals.
The individuals in different ages have characteristic gut microbes. The changes in community structure of gut microbes may be related to age-induced diseases.