盐度是海水养殖中重要的环境胁迫因子。虾肠拥有密集和多样化的微生物群落,在盐度胁迫下保持宿主健康和抗病原体能力。在这项研究中,使用16s扩增子和转录组测序技术对低盐度胁迫(15ppt)下的中国对虾肠道进行了分析。本研究旨在探讨肠道菌群和基因表达对急性低盐度应激的响应机制。使用16S微生物区系和转录组测序分析了F.chinensis的肠组织。微生物群分析表明,光细菌和弧菌的相对丰度显著下降,而Shewanella,假单胞菌,乳酸菌,Ralstonia,Colwellia,Cohaesibacter,Fusibacter,落叶松科NK4A136群成为主要群落。转录组测序鉴定了许多差异表达的基因(DEGs)。DEGs被聚集成许多基因本体论术语,并进一步丰富了一些免疫或代谢相关的京都百科全书的基因和基因组途径,包括各种类型的N-聚糖生物合成,氨基酸糖和核苷酸糖代谢,溶酶体和脂肪酸代谢.微生物群与DEGs的相关性分析表明,假单胞菌,Ralstonia,Colwellia,Cohaesibacter与免疫相关基因如peritrophin-1-like和mucin-2-like,与caspase-1样基因呈负相关。低盐度胁迫引起肠道微生物及其基因表达的变化,它们之间有着密切的关联。
Salinity is an important environmental stress factor in mariculture. Shrimp intestines harbor dense and diverse microbial communities that maintain host health and anti-pathogen capabilities under salinity stress. In this study, 16s amplicon and transcriptome sequencing were used to analyze the intestine of Fenneropenaeus chinensis under low-salinity stress (15 ppt). This study aimed to investigate the response mechanisms of the intestinal microbiota and gene expression to acute low-salinity stress. The intestinal tissues of F. chinensis were analyzed using 16S microbiota and transcriptome sequencing. The microbiota analysis demonstrated that the relative abundances of Photobacterium and Vibrio decreased significantly, whereas Shewanella, Pseudomonas, Lactobacillus, Ralstonia, Colwellia, Cohaesibacter, Fusibacter, and Lachnospiraceae_NK4A136_group became the predominant communities. Transcriptome sequencing identified numerous differentially expressed genes (DEGs). The DEGs were clustered into many Gene Ontology terms and further enriched in some immunity- or metabolism-related Kyoto Encyclopedia of Genes and Genomes pathways, including various types of N-glycan biosynthesis, amino acid sugar and nucleotide sugar metabolism, and lysosome and fatty acid metabolism. Correlation analysis between microbiota and DEGs showed that changes in Pseudomonas, Ralstonia, Colwellia, and Cohaesibacter were positively correlated with immune-related genes such as peritrophin-1-like and mucin-2-like, and negatively correlated with caspase-1-like genes. Low-salinity stress caused changes in intestinal microorganisms and their gene expression, with a close correlation between them.