■作为肿瘤的一员,皮肤黑素瘤(SKCM)因其恶性程度高,对人类健康构成严重威胁。不幸的是,目前仍缺乏有效的SKCM治疗方法。FANCI在各种肿瘤的发生和转移中起着至关重要的作用。然而,其在SKCM中的调节作用尚不清楚。本研究的目的是探讨FANCI与SKCM的相关性。
■本研究在R环境中使用“limma”或“DESeq2”包,从基因表达综合数据库和癌症基因组图谱(TCGA)-SKCM数据集中调查了GSE46517,GSE15605和GSE114445中FANCI的表达,并利用GEPIA数据库调查了FANCI的预后意义。此外,我们的研究使用实时定量聚合酶链反应(RT-qPCR)和免疫组织化学(IHC)染色来验证SKCM与正常组织之间的FANCI表达,并在A375和A875细胞中进行FANCI的敲减,以进一步分析FANCI的功能。最后,本研究通过CIBERSORT分析FANCI与肿瘤浸润免疫细胞的相关性,估计,和ssGSEA算法。
■来自GSE46517、GSE15605、GSE114445和TCGA-SKCM的SKCM组织中FANCI水平升高。然而,高FANCI表达与低总生存率相关。RT-qPCR和IHC证实了生物信息学的准确性。抑制FANCI抑制A375和A875细胞增殖,迁移,和入侵。FANCI可通过调节免疫反应和浸润众多免疫细胞参与SKCM的免疫环境。特别是中性粒细胞,CD8+T细胞,B细胞。此外,据报道,具有高FANCI表达水平的SKCM患者表现出免疫抑制,而FANCI表达水平较低的患者更有可能从免疫治疗中获得积极结果.
■SKCM中增加的FANCI表达可能是预后生物标志物。敲除FANCI可以减少SKCM的发生和进展。FANCI的表达为预测SKCM的免疫状态和治疗提供了基础。
As a member of tumor, Skin cutaneous melanoma (SKCM) poses a serious threat to people\'s health because of its strong malignancy. Unfortunately, effective treatment methods for SKCM remain lacking. FANCI plays a vital role in the occurrence and metastasis of various tumor types. However, its regulatory role in SKCM is unclear. The purpose of this study was to explore the association of FANCI with SKCM.
This study investigated the expression of FANCI in GSE46517, GSE15605, and GSE114445 from the Gene Expression Omnibus database and The Cancer Genome Atlas (TCGA)-SKCM datasets using the package \"limma\" or \"DESeq2\" in R environment and also investigated the prognostic significance of FANCI by utilizing the GEPIA database. Additionally, our research made use of real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemical (IHC) staining to verify FANCI expression between SKCM and normal tissues and developed the knockdown of FANCI in A375 and A875 cells to further analyze the function of FANCI. Finally, this study analyzed the correlation of FANCI and tumor-infiltrating immune cells by CIBERSORT, ESTIMATE, and ssGSEA algorithms.
The FANCI level was increasing in SKCM tissues from GSE46517, GSE15605, GSE114445, and TCGA-SKCM. However, high FANCI expression correlated with poor overall survival. The RT-qPCR and IHC confirmed the accuracy of bioinformatics. Knocking down FANCI suppresses A375 and A875 cell proliferation, migration, and invasion. FANCI could be involved in the immunological milieu of SKCM by regulating immune responses and infiltrating numerous immune cells, particularly neutrophils, CD8+ T cells, and B cells. Furthermore, patients with SKCM who have a high FANCI expression level are reported to exhibit immunosuppression, whereas those with a low FANCI expression level are more likely to experience positive outcomes from immunotherapy.
The increased FANCI expression in SKCM can be a prognostic biomarker. Knockdown FANCI can reduce the occurrence and progression of SKCM. The FANCI expression provides a foundation for predicting the immune status and treatment of SKCM.