皮肤鳞状细胞癌(cSCC)是一种常见且日益普遍的皮肤癌,带来重大的健康挑战。了解cSCC进展中涉及的分子机制对于开发有效的治疗至关重要。这项研究的主要目的是通过逐步化学过程评估NOTCH1和FGFR2癌基因在诱导FVB/N小鼠皮肤癌中的激活。40只雌性FVB/N小鼠,四周大,随机分为对照组(n=8)和两个实验组(A组:n=16,B组:n=16)。这项研究涉及对这些群体进行两阶段致癌程序。这包括在其背部剃须的皮肤上初始应用97.4nmolDMBA,A组13周后应用32.4nmolTPA,B组20周后应用TPA。对照组不接受任何治疗。每周监测他们的皮肤状况以检测肿瘤发展。实验之后,将动物安乐死用于进一步的组织取样。实验组皮肤病变的检查显示与肿瘤进展有关,从发育不良到癌症。对肿瘤样品进行组织学和免疫组织化学评估。值得注意的是,良性组织中FGFR2表达较高,癌前病变,与正常组织相比,恶性肿瘤。与正常组织相比,NOTCH1表达仅在良性肿瘤中升高。这项研究表明FGFR2表达与皮肤肿瘤的进展有明显的相关性。而NOTCH1在FVB/N小鼠中的表达呈负相关。这表明这些癌基因早期参与皮肤肿瘤的发展。
Cutaneous squamous cell carcinoma (cSCC) is a common and increasingly prevalent form of skin cancer, posing significant health challenges. Understanding the molecular mechanisms involved in cSCC progression is crucial for developing effective treatments. The primary aim of this research was to evaluate the activation of NOTCH1 and FGFR2 oncogenes in inducing skin cancer in FVB/N mice through a stepwise chemical process. Forty female FVB/N mice, aged four weeks, were randomly divided into a control group (n = 8) and two experimental groups (group A: n = 16, group B: n = 16). This study involved subjecting the groups to a two-stage carcinogenesis procedure. This included an initial application of 97.4 nmol DMBA on shaved skin on their backs, followed by applications of 32.4 nmol TPA after thirteen weeks for group A and after twenty weeks for group B. The control group did not receive any treatment. Their skin conditions were monitored weekly to detect tumor development. After the experiment, the animals were euthanized for further tissue sampling. The examination of skin lesions in the experimental groups showed a correlation with tumor progression, ranging from dysplasia to carcinoma. Tumor samples were assessed both histologically and immunohistochemically. Notably, FGFR2 expression was higher in benign, precancerous, and malignant tumors compared to normal tissue. NOTCH1 expression was only elevated in benign tumors compared to normal tissue. This study demonstrates a clear correlation of FGFR2 expression and the progression of cutaneous neoplasms, while NOTCH 1 expression is inversely correlated in FVB/N mice. This suggests an early involvement of these oncogenes in the development of skin tumors.