■可注射的皮肤填充物为皮肤抗衰老和面部嫩肤提供了更广泛的选择。PLLA微球作为可降解和持久的填料越来越受欢迎。本研究仅关注PLLA对真皮胶原蛋白的影响。没有调查它对表皮的影响。在这项研究中,我们研究了PLLA微球对表皮干细胞(EpiSCs)的影响。
■通过体外培养不同浓度的PLLA微球对表皮干细胞(EpiSCs)的作用,原代大鼠EpiSCs的鉴定。CCK-8检测,凋亡染色,流式细胞术,Transwell分析,伤口愈合试验,q-PCR分析,免疫荧光染色检测PLLA对EpiSCs的影响。此外,我们观察到通过体内将PLLA注射到大鼠皮肤的真皮中对表皮的影响。
■PLLA微球促进细胞增殖和迁移,同时延缓细胞衰老并保持其干性。体外,大鼠背部皮肤皮内注射PLLA微球导致延缓衰老,随访2、4和12周皮肤的组织学和免疫组织化学染色证明了这一点。
■这项研究表明PLLA对大鼠表皮和EpiSCs的积极作用,同时为PLLA的抗衰老机制提供了新的见解。
UNASSIGNED: Injectable skin fillers offer a wider range of options for cutaneous anti-aging and facial rejuvenation. PLLA microspheres are increasingly favored as degradable and long-lasting fillers. The present study focused solely on the effect of PLLA on dermal collagen, without investigating its impact on the
epidermis. In this study, we investigated the effects of PLLA microspheres on epidermal stem cells (EpiSCs).
UNASSIGNED: Different concentrations of PLLA microspheres on epidermal stem cells (EpiSCs) in vitro through culture, and identification of primary rat EpiSCs. CCK-8 detection, apoptosis staining, flow cytometry, Transwell assay, wound healing assay, q-PCR analysis, and immunofluorescence staining were used to detect the effects of PLLA on EpiSCs. Furthermore, we observed the effect on the
epidermis by injecting PLLA into the dermis of the rat skin in vivo.
UNASSIGNED: PLLA microspheres promote cell proliferation and migration while delaying cell senescence and maintaining its stemness. In vitro, Intradermal injection of PLLA microspheres in the rat back skin resulted in delayed aging, as evidenced by histological and immunohistochemical staining of the skin at 2, 4, and 12 weeks of follow-up.
UNASSIGNED: This study showed the positive effects of PLLA on rat
epidermis and EpiSCs, while providing novel insights into the anti-aging mechanism of PLLA.