在本文中,我们展示了急性低剂量中子对大鼠全身的生物学效应,并研究了该水平的中子剂量对诱导体内放射适应性反应的影响.要了解无线电自适应响应,被检查的动物暴露于5和10mSv的急性中子辐射剂量,14天后给予50mSv攻击剂量。辐照后,所有接受单剂量和双剂量的组在取样前都在笼子里呆一天.电子顺磁共振(EPR)方法用于估计血液中辐射诱导的自由基,并测定了一些血液学参数和脂质过氧化(MDA)。在一些抗氧化酶[过氧化氢酶(CAT),超氧化物歧化酶(SOD),和谷胱甘肽(GSH)]。根据中子暴露剂量对七组成年雄性大鼠进行分类。在收获后一周进行所有研究标记的测量,除了血液学标志物,在2小时内。结果表明抗氧化酶的产量较低(CAT降低了1.18-5.83%,SOD由1.47-17.8%,和GSH增长11.3-82.1%)。此外,红细胞分布宽度(RDW)增加(从4.61增加到25.19%)和彗星测定参数,如尾部长度,(从6.16到10.81µm),尾部力矩,(从1.17到2.46µm),在所有暴露于5至50mSv急性剂量辐射的组中,尾部长度(DNA%)的DNA百分比(从9.58到17.32%),分别。这强调了随着急性热中子剂量的增加而增加的有害作用。所研究的所有标记物的放射自适应响应的引入因子的值表明,较低的引发剂量促进较高的适应响应,反之亦然。最终,结果表明DNA%存在显著差异,SOD酶水平,EPR强度,总Hb浓度,和RDW,提示它们作为急性热中子剂量学生物标志物的潜在用途。需要进一步的研究来验证这些测量作为辐射暴露的生物剂量学,包括涉及不同攻击剂量和辐照后行为的RAR响应影响的调查。
In this paper, we demonstrated the biological effects of acute low-dose neutrons on the whole body of rats and investigated the impact of that level of neutron dose to induce an in vivo radio-adaptive response. To understand the radio-adaptive response, the examined animals were exposed to acute neutron radiation doses of 5 and 10 mSv, followed by a 50 mSv challenge dose after 14 days. After irradiation, all groups receiving single and double doses were kept in cages for one day before sampling. The electron paramagnetic resonance (EPR) method was used to estimate the radiation-induced radicals in the blood, and some hematological parameters and lipid peroxidation (MDA) were determined. A comet assay was performed beside some of the antioxidant enzymes [catalase enzyme (CAT), superoxide dismutase (SOD), and glutathione (GSH)]. Seven groups of adult male rats were classified according to their dose of neutron exposure. Measurements of all studied markers are taken one week after harvesting, except for hematological markers, within 2 h. The results indicated lower production of antioxidant enzymes (CAT by 1.18-5.83%, SOD by 1.47-17.8%, and GSH by 11.3-82.1%). Additionally, there was an increase in red cell distribution width (RDW) (from 4.61 to 25.19%) and in comet assay parameters such as Tail Length, (from 6.16 to 10.81 µm), Tail Moment, (from 1.17 to 2.46 µm), and percentage of DNA in tail length (DNA%) (from 9.58 to 17.32%) in all groups exposed to acute doses of radiation ranging from 5 to 50 mSv, respectively. This emphasizes the ascending harmful effect with the increased acute thermal neutron doses. The values of the introduced factor of radio adaptive response for all markers under study reveal that the lower priming dose promotes a higher adaptation response and vice versa. Ultimately, the results indicate significant variations in DNA%, SOD enzyme levels, EPR intensity, total Hb concentration, and RDWs, suggesting their potential use as biomarkers for acute thermal neutron
dosimetry. Further research is necessary to validate these measurements as biodosimetry for radiation exposure, including investigations involving the response impact of RAR with varied challenge doses and post-irradiation behavior.