卢旺达是非洲最小的国家之一,在那里,法医遗传研究很少进行,DNA数据库也很少开发。使用HUMDNATYYhuang(Yanhuang)试剂盒在505个无关的卢旺达人中研究了短串联重复序列(STR)多态性。目标为下列STR:D3S1358,D13S317,D7S820,D16S539,SE33,D10S1248,D5S818,D21S11,TPOX,D1S1656,D6S1043,D19S433,D22S1045,D8S1179,PentaE,D2S441,D12S391,D2S1338,vWA,PentaD,TH01、D18S51、CSF1PO和FGA。这项研究的目的是阐明遗传多样性,并探索在505名卢旺达人口中应用这24种STR的潜力。共有360个等位基因,在卢旺达人群中发现相应的等位基因频率在0.001至0.442之间。SE33在这24个基因座中表现出最高的多态性(PIC=0.921),而D13S317显示最低(PIC=0.671)。对于24个基因座中的任何一个,均未观察到Hardy-Weinberg平衡的偏差。法医参数,包括歧视的综合权力(PD和综合排斥权力,已经证明,这个由24个可疑交易报告组成的小组提供了很高的信息,对诸如个人身份识别和亲子鉴定等法医应用很有用。此外,根据8个重叠基因座计算了卢旺达种群与其他24个已发表种群之间的遗传距离,多遗传树揭示了种群中与其地理位置和历史关系相关的显着簇。
Rwanda is one of the smallest countries of Africa, where forensic genetic studies are rarely being conducted and very few DNA databases have been developed. Short tandem repeats (STRs) polymorphisms were investigated in 505 unrelated Rwandese by using the HUMDNA TYPING (Yanhuang) Kit. The following STRs were targeted: D3S1358, D13S317, D7S820, D16S539, SE33, D10S1248, D5S818, D21S11, TPOX, D1S1656, D6S1043, D19S433, D22S1045, D8S1179, Penta E, D2S441, D12S391, D2S1338, vWA, Penta D, TH01, D18S51, CSF1PO and FGA. The purpose of this study was to elucidate the genetic diversity and explore the potential of applying these 24 STR in 505 Rwandan population in forensics. A total of 360 alleles, with corresponding allele frequencies in the range from 0.001 to 0.442, were found in the Rwandan population. SE33 presented the highest polymorphism (PIC=0.921) among these 24 loci, whereas D13S317 presented the lowest one (PIC=0.671). No deviation from the Hardy-Weinberg equilibrium was observed for any of the 24 loci. The forensic parameters, including the combined power of discrimination (PD and the combined exclusion power, have demonstrated that this panel of 24 STRs is highly informative and useful for forensic applications such as individuals\' identification and paternity tests. Additionally, the genetic distances between Rwanda population and other 24 published populations were calculated based on 8 overlapping loci with the polygenetic tree revealing significant clusters in the populations associated with their geographic locations and their historical relationship.