■用于角膜炎-角膜交联的光活化发色团(PACK-CXL)稳定了角膜基质并消除了微生物。许多PACK-CXL协议,使用不同的能源和发色团,已应用于临床前研究,包括活体动物研究,具有各种实验设计和终点。到目前为止,应用方案的系统映射和跨研究的一致性似乎缺乏,但对于指导未来的研究至关重要.
■范围审查方案符合JBI证据综合手册。搜索了电子数据库(Embase,MEDLINE,Scopus,WebofScience)以识别合格的记录,接下来是两步选择过程(标题和摘要筛选,全文筛选)用于记录。我们提取了以下信息:(1)不同的PACK-CXL方案特征;(2)测试的感染性病原体;(3)研究设计和实验设置;(4)用于确定抗菌和组织稳定作用的终点。这些信息被绘制在频率图中。
■搜索产生了3654条唯一记录,其中233项符合纳入标准。有103个异构端点,研究人员调查了广泛的PACK-CXL协议.测试的微生物反映了通常与感染性角膜炎相关的病原体。细菌溶液和感染性角膜炎兔模型是研究PACK-CXL抗菌作用的最广泛使用的模型。
■如果PACK-CXL临床前研究指导未来的转化研究,需要进一步的跨学科努力来建立,promote,并促进与PACK-CXL相关的共同端点的接受。
■临床前研究中PACK-CXL方案的系统作图指导未来的转化研究。
UNASSIGNED: Photoactivated chromophore for keratitis-corneal cross-linking (PACK-CXL) stabilizes the corneal stroma and eliminates microorganisms. Numerous PACK-CXL protocols, using different energy sources and chromophores, have been applied in preclinical studies, including live animal studies, with various experimental designs and endpoints. So far, a systematic mapping of the applied protocols and consistency across studies seems lacking but is essential to guide future research.
UNASSIGNED: The scoping review protocol was in line with the JBI Manual for Evidence Synthesis. Electronic databases were searched (Embase, MEDLINE, Scopus, Web of Science) to identify eligible records, followed by a two-step selection process (title and abstract screening, full text screening) for record inclusion. We extracted information on (1) different PACK-CXL protocol characteristics; (2) infectious pathogens tested; (3) study designs and experimental settings; and (4) endpoints used to determine antimicrobial and tissue stabilizing effects. The information was charted in frequency maps.
UNASSIGNED: The searches yielded 3654 unique records, 233 of which met the inclusion criteria. With 103 heterogeneous endpoints, the researchers investigated a wide range of PACK-CXL protocols. The tested microorganisms reflected pathogens commonly associated with infectious keratitis. Bacterial solutions and infectious keratitis rabbit models were the most widely used models to study the antimicrobial effects of PACK-CXL.
UNASSIGNED: If preclinical PACK-CXL studies are to guide future translational research, further cross-disciplinary efforts are needed to establish, promote, and facilitate acceptance of common endpoints relevant to PACK-CXL.
UNASSIGNED: Systematic mapping of PACK-CXL protocols in preclinical studies guides future translational research.