■由于它们对各种分析物的敏感和选择性反应,近年来,比色传感器已获得广泛接受。金纳米粒子(AuNP)由于其高稳定性和易用性而被广泛用于视觉传感器策略。将AuNP与响应性聚合物组合可以导致不同的表面等离子体共振(SPR)变化,其可以用作比色生物传感器。
■这项研究的目的是通过利用与3-氨基苯基硼酸(APBA)共轭的pH响应聚合物聚(丙烯酸)(PAA)交联的金纳米颗粒(AuNP)的光学性质来开发基于比色的传感器。
■通过RAFT聚合合成聚合物(PAA)。反向Turkevic方法用于产生AuNPs,随后将其用于使用聚(丙烯酸)-氨基苯基硼酸(PAA-APBA)的自组装过程中,以创建自组装的AuNP-APBA-PAA。颗粒大小,zeta电位,使用透射电子显微镜(TEM)确定聚合物修饰的金纳米颗粒的可逆性,粒度分析仪(PSA),和紫外-可见分光光度计(UV-Vis分光光度计)。视觉,UV-Vis分光光度计和TEM观察证实了该系统识别细菌的能力。使用Excel软件使用单向方差分析进行统计学分析。
■使用紫外-可见分光光度法,AuNP的粒径确定为25.7nm,最大吸光度出现在530nm。AuNPsPAAAPBA胶体显示出吸光度最大值为532nm,zeta电位为-41.53,pH转变点在4和5之间。在大肠杆菌浓度为4.5×107CFU/mL时,孵育15小时后,系统传感器的颜色从红色变为蓝色,而在金黄色葡萄球菌浓度为1.2×109CFU/mL时,混合后立即变为紫色。TEM证实,检测机制是基于大肠杆菌和金黄色葡萄球菌外膜上糖类的硼酸酯-多元醇键。
■将APBA与包含AuNPs的pH响应性PAA聚合物结合使用,以检测大肠杆菌和金黄色葡萄球菌细菌诱导最大波长转变,颜色从红色变为蓝色。通过响应性聚合物的去溶胀过程,诱导AuNP的聚集,建立的传感器系统能够改变颜色。基于共轭聚合物和金纳米颗粒的传感器系统展示了一种有前途的细菌检测方法。
UNASSIGNED: Because of their sensitive and selective responses to a wide variety of analytes, colorimetric sensors have gained widespread acceptance in recent years. Gold nanoparticles (AuNPs) are widely employed in visual sensor strategies due to their high stability and ease of use. Combining AuNPs with a responsive polymer can result in distinct surface plasmon resonance (SPR) changes that can be utilized as colorimetric biosensors.
UNASSIGNED: The purpose of this research is to develop a colorimetric-based sensor through the utilization of the optical properties of gold nanoparticles (AuNPs) crosslinked with pH-responsive polymers poly (acrylic acid) (PAA) conjugated to 3-aminophenyl boronic acid (APBA).
UNASSIGNED: The polymer (PAA) was synthesized via RAFT polymerization. The inversed Turkevic method was used to produce AuNPs, which were subsequently used in a self-assembly process using poly (acrylic acid)-aminophenyl boronic acid (PAA-APBA) to create the self-assembled AuNPs-APBA-PAA. The particle size, zeta potential, and reversibility of the polymer-modified gold nanoparticles were determined using a transmission electron microscope (TEM), a particle size analyzer (PSA), and an Ultraviolet-Visible spectrophotometer (UV-Vis spectrophotometer). Visual, UV-Vis spectrophotometer and TEM observations confirmed the system\'s ability to identify bacteria. Statistical analysis was performed using a one-way analysis of variance using Excel software.
UNASSIGNED: Using UV-Vis spectrophotometry, the particle size of AuNPs was determined to be 25.7 nm, and the maximum absorbance occurred at 530 nm. AuNPs PAA APBA colloid exhibited an absorbance maximum of 532 nm, a zeta potential of -41.53, and a pH transition point between 4 and 5. At E. coli concentrations of 4.5 x 107 CFU/mL, the color of the system sensors changed from red to blue after 15 hours of incubation, whereas at S. aureus concentrations of 1.2 x 109 CFU/mL, the color changed to purple immediately after mixing. The TEM confirmed that the detection mechanism is based on the boronate-polyol bonding of saccharides on the outer membranes of Escherichia coli and Staphylococcus aureus.
UNASSIGNED: The use of APBA in conjunction with pH-responsive PAA polymers containing AuNPs to detect E. coli and S. aureus bacteria induces a maximum wavelength transition, followed by a color change from red to blue. By the process of de-swelling of the responsive polymer, which induces the aggregation of the AuNPs, the established sensor system is able to alter the color. The conjugated polymer and gold nanoparticle-based sensor system demonstrated a promising method for bacterial detection.