■大多数现代血液学分析仪具有用于体液细胞计数的专用体液模式。许多分析仪还计算高荧光细胞(HF细胞)的数量。HF细胞具有大的核尺寸并且在用荧光染料染色时发射高荧光。由于它们的核尺寸很大,恶性细胞计数为HF细胞。
■我们的目标是确定HF细胞在预测浆液性积液中恶性细胞的存在中的诊断实用性。
■使用MindrayBC-6800加血液学分析仪的体液模式对209个浆液样品进行HF细胞计数。一组细胞病理学家检查了所有样品的Papanicilaou染色涂片中是否存在恶性细胞。ROC曲线分析确定HF细胞在恶性积液中的敏感性和特异性。
■在209个样品中,显微镜下发现恶性细胞97例(46.4%)。恶性积液中HF细胞的绝对数量和百分比显着增加(P<0.001)(HF#=24.9细胞/ul,HF%=10.4%)与非恶性样品(HF#=4.95细胞/ul,HF%=5.76%)。ROC曲线分析确定了预测恶性细胞的最佳临界值≥30个HF细胞/ul(灵敏度=73.91,特异性=55.66%)。
■浆液性积液中的HF细胞可能是帮助病理学家的有用工具,但由于其敏感性低(67.74%)和阴性似然比(0.5),在≥30个HF细胞/ul的临界值下,它不是理想的筛查测试.然而,由于在≥72个HF细胞/ul的临界值下具有83.18%的高特异性,应在显微镜下仔细搜索恶性细胞。
UNASSIGNED: Most modern haematology analysers have a dedicated body fluid mode for cell counts of body fluids. Many analysers also count the number of high fluorescence cells (HF cells). HF cells have a large nuclear size and emit high fluorescence when stained with fluorescent dyes. Due to their large nuclear size, Malignant cells are counted as HF cells.
UNASSIGNED: We aim to determine the diagnostic utility of HF cells in predicting the presence of malignant cells in serous effusions.
UNASSIGNED: HF cell counts were done on 209 serous fluid samples using the body fluid mode of Mindray BC-6800 plus haematology analyser. Papanicilaou-stained smears of all samples were examined for the presence of malignant cells by a panel of cytopathologists. ROC curve analysis was done to determine the sensitivity and specificity of HF cells in malignant effusions.
UNASSIGNED: Out of 209 samples, malignant cells were found by microscopy in 97 cases (46.4%). The absolute number and percentage of HF cells were significantly higher (P < 0.001) in malignant effusions (HF# = 24.9 cells/ul, HF% = 10.4%) when compared to non-malignant samples (HF# = 4.95 cells/ul, HF% = 5.76%). ROC curve analysis determined an optimal cut-off of ≥30 HF cells/ul (sensitivity = 73.91, specificity = 55.66%) for the prediction of malignant cells.
UNASSIGNED: HF cells in serous effusions can be a helpful tool to aid the pathologist, but it is not an ideal screening test due to its low sensitivity (67.74%) and negative likelihood ratio (0.5) at a cut-off of ≥30 HF cells/ul. However, due to high specificity of 83.18% at a cut-off of ≥72 HF cells/ul, a meticulous search for malignant cells should be done on microscopy.