尖孢镰刀菌是蓝莓根腐病的主要病原,我们发现镰刀菌也会导致蓝莓根腐病。木霉属。广泛用于控制植物病害。我们从蓝莓根际土壤中分离了木霉菌（TM11），以探讨其对尖孢菌和公社的防治效果和机理。我们发现，TM11挥发物和肉汤代谢产物对尖孢酵母的抑制作用是显著的，但只有F.commune挥发性代谢产物对其生长有显著的抑制作用。通过HPLC-MS从TM11发酵液的甲醇提取物中检测到12种已知的抗菌代谢产物。TM11裂解并盘绕在尖孢镰刀菌和公社的菌丝周围。盆栽试验表明，TM11对尖孢酵母和福尔具有显著的防治效果，在尖孢梭菌和公社之前接种TM11更有效。TM11，TM11和尖孢酵母，或F.commune和蒸馏水处理对超氧化物歧化酶活性有不同的影响，过氧化物酶和过氧化氢酶，酶活性水平表现出以下顺序：TM11>TM11和F.尖孢或F.commune>蒸馏水。结果表明，TM11对蓝莓根腐病具有有效的防治作用。
Fusarium oxysporum is the primary pathogen of blueberry root rot; furthermore, we found that Fusarium commune can also cause root rot in blueberries. Trichoderma spp. is widely used to control plant diseases. We isolated Trichoderma asperellum (TM11) from blueberry rhizosphere soil to explore its control effect and mechanism on F. oxysporum and F. commune. We found that the inhibitory effects of TM11 volatiles and broth metabolites on F. oxysporum were significant, but only F. commune volatile metabolites had a significant inhibitory effect on its growth. Twelve known antimicrobial metabolites were detected from the methanol extract of TM11 fermentation broth by HPLC-MS. TM11 lysed and coiled around the hyphae of F. oxysporum and F. commune. The pot experiment showed that TM11 had significant control effects against F. oxysporum and F. commune, and inoculation of TM11 prior to that of F. oxysporum and F. commune was more effective. The TM11, TM11 and F. oxysporum, or F. commune and distilled water treatments had different effects on the activities of superoxide dismutase, peroxidase and catalase, and the enzyme activity levels exhibited the following order: TM11 > TM11 and F. oxysporum or F. commune > distilled water. The results showed that TM11 provided effective control of blueberry root rot.
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