类风湿性关节炎(RA)中免疫复合物的功能与其组成和大小有关。使用动态光散射(DLS),我们调查了RA循环免疫复合物(CIC)颗粒大小与CIC免疫球蛋白水平之间的联系.在这项研究中,纳入30例RA患者和30例健康个体。IgA,IgG,在所有分析的CICs中都发现了IgM,但RA中的IgA和IgG含量高于对照组。在对照和RACI中,DLS检测到50个大小不同的颗粒,并聚集在两个大小组周围:半径为7.5-164nm,半径为342-1718nm。RACICs中IgA水平升高,与对照组相比,与超过50%的CIC颗粒相关。在RA中,与对照相比,具有28.2nm的较高数量的CICs,531nm,712nm,检测到1718nm颗粒和较低数量的具有78.8nm颗粒的CICs。这种颗粒分布模式不能反映CIC免疫球蛋白水平的变化。因此,RA升高的CICIgA与所有这些颗粒(1718nm颗粒除外)连接,IgM的增加与43.8nm和712nm颗粒有关,并且IgG的增加仅与712nm颗粒有关。这项研究提供了关于CIC颗粒大小之间关联的第一个数据,CIC免疫球蛋白水平,RA。在大规模研究确认后,通过DLS确定的CICs大小可以用作RA诊断或监测的标准。
The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles\' size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5-164 nm radius and with a 342-1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles\' size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.