背景:纳米技术能够在分子水平上精确操纵物质,纳米粒子在医学和其他领域提供了不同的应用。绿色合成方法,利用植物提取物等天然来源,因其生态友好而受到青睐。氧化锌(ZnO)纳米颗粒以其对抗微生物和减少炎症的能力而闻名,这为生物医学应用带来了希望。长春花,以其药用特性而闻名,由于其抗炎和抗氧化特性,值得在口腔健康管理方面进一步探索。
目的:本研究旨在合成长春花介导的ZnO纳米颗粒,并评估其抗炎和抗氧化活性。
方法:将长春花粉(1g)溶解在蒸馏水(100ml)中,在60°C下加热15-20分钟,过滤,得到20ml提取物。通过将0.594gZnO粉末添加到50ml水中来合成ZnO纳米颗粒,与植物提取物混合,搅拌72小时,并将所得溶液离心。使用Bruker的AlphaIIFTIR光谱仪(Bruker,Billerica,马萨诸塞州,美国),抗氧化剂,和抗炎活性。
结果:FTIR分析揭示了长春花介导的ZnO纳米颗粒中存在的官能团的特征峰,包括O-H,N-O,C-O,C=C,和C=C-H抗炎活性评价显示抑制范围从48%到89%,最大抑制在50μL浓度。同样,抗氧化活性范围从62%到88%,在50μL浓度下也观察到最大抑制。
结论:两种测定均有效地展示了与对照相比,长春花掺入的ZnO纳米颗粒提取物的优异的抗炎和抗氧化活性。这表明它们作为进一步评估的可行治疗剂的潜力。
BACKGROUND: Nanotechnology enables precise manipulation of matter at the molecular level, with nanoparticles offering diverse applications in medicine and beyond. Green synthesis methods, utilizing natural sources like plant extracts, are favored for their eco-friendliness. Zinc oxide (ZnO) nanoparticles are recognized for their ability to combat microbes and reduce inflammation, which holds promise for biomedical applications. Catharanthus roseus, renowned for its medicinal properties, warrants further exploration in oral health management due to its anti-inflammatory and antioxidant attributes.
OBJECTIVE: The current study aimed to synthesize Catharanthus roseus-mediated ZnO nanoparticles and to evaluate their anti-inflammatory and antioxidant activity.
METHODS: Catharanthus roseus powder (1 g) was dissolved in distilled water (100 ml), heated at 60°C for 15-20 minutes, and filtered to obtain 20 ml extract. ZnO nanoparticles were synthesized by adding 0.594 g ZnO powder to 50 ml water, mixed with plant extract, and stirred for 72 hours, and the resulting solution was centrifuged. Nanoparticles were collected and analyzed for Fourier-transform infrared spectroscopy (FTIR) using Bruker\'s Alpha II FTIR spectrometer (Bruker, Billerica, Massachusetts, United States), antioxidant, and anti-inflammatory activities.
RESULTS: FTIR analysis revealed characteristic peaks indicative of functional groups present in Catharanthus roseus-mediated ZnO nanoparticles, including O-H, N-O, C-O, C=C, and C≡C-H. Anti-inflammatory activity evaluation showed inhibition ranging from 48% to 89%, with the maximum inhibition at 50 μL concentration. Similarly, antioxidant activity ranged from 62% to 88%, with the maximum inhibition also seen at 50 μL concentration.
CONCLUSIONS: Both assays effectively showcased the superior anti-inflammatory and antioxidant activity of the Catharanthus roseus-incorporated ZnO nanoparticles extract compared to the control. This suggests their potential as a viable therapeutic agent for further evaluation.