人类李斯特菌病的爆发通常与食用受污染的食物有关，尤其是肉制品。为了更好地了解单核细胞增生李斯特菌的肉类污染，对所有检测到的分离株进行全基因组测序(WGS),以调查零售市场和屠宰场之间的遗传关系.分别从1914个食品样本和67个食品和环境样本中分离出110个和13个分离株，分别。IIa（51/123,41.5％）和IIc（7/123,5.7％）被检测为123个单核细胞增生性大肠杆菌分离株的主要血清群。大多数分离株在表型试验中对青霉素耐药(22/123,17.9%),所有分离株也被发现对氨苄青霉素敏感，美罗培南,和万古霉素.在35个菌株中，所有这些菌株都具有与毒力相关的基因和inlA基因中的过早终止密码子（PMSCs）。鉴定了22种多位点序列类型和19种克隆复合物，最常见的是ST9。这项研究还显示了吉林菌株的患病率和独特性，中国与世界范围内流行的国际毒株比较。这项研究的结果将有助于对中国北方地区生产和流通中单核细胞增生李斯特菌传播的流行病学认识。
Human listeriosis outbreaks are often associated with consumption of contaminated food, especially meat products. To better understand meat contamination of L. monocytogenes, whole genome sequencing(WGS) was performed on all detected isolates to investigate genetic relationships between retail markets and slaughterhouses. 110 and 13 isolates were isolated from 1914 food samples and 67 food and environmental samples, respectively. IIa (51/123,41.5%) and IIc (7/123,5.7%) were detected as the dominant serogroups of 123 L. monocytogenes isolates.Most isolates were penicillin-resistant (22/123,17.9%) in the phenotypic test, and all isolates were also found to be susceptible to ampicillin, meropenem, and vancomycin. All of them harbored virulence-associated genes and premature stop codons (PMSCs) in inlA genes were occurred in 35 strains. 22 multilocus sequence types and 19 clonal complexes were identified with ST9 being most common. This study also showed the prevalence and uniqueness of strains from Jilin,
China compared with worldwide epidemic international strains. The findings of this study will contribute to the epidemiological understanding of transmission of L. monocytogenes from production and circulation in the region of northern
China.
