小滴数字PCR(ddPCR)最近已被证明是成人血液感染(BSIs)的潜在诊断工具;然而,它在儿童中的应用仍然不清楚。在这项研究中,通过传统血液培养(BCs)和ddPCRs同步检测了76例可疑BSI儿童的血液样本。我们的团队验证了ddPCR的诊断性能,包括灵敏度,特异性,以及阳性和阴性预测值。来自血液科的76例儿科患者(67.1%),儿科重症监护病房(PICU,27.6%),和其他部门(5.2%)登记。ddPCR结果阳性率为47.9%,而BC为6.6%。此外,ddPCR的时间消耗更短,仅适用于4.7±0.9h,与BC的检测定时(76.7±10.4h,p<0.01)。BC和ddPCR之间的一致和分歧水平分别为96.1%和4.2%,消极协议达到95.6%。ddPCR的灵敏度为100%,相应的特异性范围从95.3到100.0%。此外,通过ddPCR共鉴定出9种病毒。在中国,多重ddPCR首先可能是一种快速准确诊断疑似BSIs儿童的工具,并且可能是免疫抑制儿童病毒血症可能性的早期指标.
Droplet digital PCR (ddPCR) recently has been shown to be a potential diagnostic tool for adults with bloodstream infections (BSIs); however, its application in children remains obscure. In this study, 76 blood samples of children with suspected BSIs were synchronously detected by traditional blood cultures (BCs) and ddPCRs. Our team validated the diagnostic performance of ddPCR including sensitivity, specificity, and positive and negative predictive values. The 76 pediatric patients from the hematology department (67.1%), the pediatric intensive care unit (PICU, 27.6%), and other departments (5.2%) were enrolled. The positive rate of ddPCR results was 47.9%, whereas that for BC was 6.6%. In addition, the time consumption of ddPCR was shorter, only for 4.7 ± 0.9 h, in comparison with the detection timing of BC (76.7 ± 10.4 h, p < 0.01). The levels of agreement and disagreement between BC and ddPCR were 96.1% and 4.2%, and the negative agreement reached 95.6%. The sensitivity of ddPCR was 100%, with corresponding specificities ranging from 95.3 to 100.0%. In addition, a total of nine viruses were identified by ddPCR. In
China, the multiplexed ddPCR first could be a tool for the rapid and accurate diagnosis of children with suspected BSIs and can be an early indicator of the possibility of
viraemia in children with immunosuppression.