马立克氏病病毒(MDV),自然致癌,高度传染性的α疱疹病毒,在鸡中诱导T细胞淋巴瘤,造成严重的经济损失。个体的Marek病(MD)结果归因于遗传和环境因素。需要进一步研究影响MD抗性的宿主-病毒相互作用机制以实现更大的MD控制。这项研究分析了2个高度近交亲本系63和72以及5个重组同基因菌株(RCS)C中的全基因组DNA甲基化模式,L,M,N,和这些父母的X株。品系63和72,具有MD抗性和易感,分别,而RCS有87.5%的63线和12.5%的72线的不同组合。我们的DNA甲基化簇显示与MD发病率密切相关。捕获亲本系和RCS之间的差异甲基化区域(DMRs)。DNA甲基化的MD抗性和MD易感标记被鉴定为可跨代表观遗传。此外,在2个亲本系和RCS中,v-srcDNA肿瘤的生长和针对绵羊红细胞的抗体反应不同。总的来说,我们的结果提供了非常可靠的证据,即DNA甲基化模式是鸡的跨代表观遗传(TEI),并且在MD肿瘤发生和其他免疫反应中也起着至关重要的作用;特定的甲基化区域可能是一般免疫的重要调节剂.
Marek\'s disease virus (MDV), a naturally oncogenic, highly contagious alpha herpesvirus, induces a T cell lymphoma in chickens that causes severe economic loss. Marek\'s disease (MD) outcome in an individual is attributed to genetic and environmental factors. Further investigation of the host-virus interaction mechanisms that impact MD resistance is needed to achieve greater MD control. This study analyzed genome-wide DNA methylation patterns in 2 highly inbred parental lines 63 and 72 and 5 recombinant congenic strains (RCS) C, L, M, N, and X strains from those parents. Lines 63 and 72, are MD resistant and susceptible, respectively, whereas the RCS have different combinations of 87.5% Line 63 and 12.5% Line 72. Our DNA methylation cluster showed a strong association with MD incidence. Differentially methylated regions (DMRs) between the parental lines and the 5 RCS were captured. MD-resistant and MD-susceptible markers of DNA methylation were identified as transgenerational epigenetic inheritable. In addition, the growth of v-src DNA tumors and antibody response against sheep red blood cells differed among the 2 parental lines and the RCS. Overall, our results provide very solid evidence that DNA methylation patterns are transgenerational epigenetic inheritance (TEI) in chickens and also play a vital role in MD tumorigenesis and other immune responses; the specific methylated regions may be important modulators of general immunity.