在农田里,对许多麻烦的杂草物种中发现的乙酰乳酸合酶(ALS)抑制除草剂的抗性,包括BromusjaponicusThunb,是一个世界性的问题。特别是,竹节虫的除草剂抗性发展严重威胁着我国的小麦生产。本研究的目的是研究日本血吸虫对氟卡巴酮钠的抗性的生理和分子基础。剂量反应分析表明,与易感的日本芽孢杆菌(S)群体相比,抗性(R)群体的氟卡巴酮钠抗性增加了120倍。ALS基因的核苷酸序列比对表明,ALS中的Pro-197-Ser突变与R群体对氟卡巴酮钠的抗性有关。马拉硫磷预处理研究的结果表明,日本血吸虫也可能具有显着的细胞色素P450单加氧酶(P450)介导的代谢抗性。这是Pro-197-Ser突变和P450介导的代谢的首次报道,该代谢赋予日本血吸虫对氟卡巴酮钠的抗性。
In crop fields, resistance to acetolactate synthase (ALS)-inhibiting herbicides found in many troublesome weed species, including Bromus japonicus Thunb, is a worldwide problem. In particular, the development of herbicide resistance in B. japonicus is a severe threat to wheat production in
China. The purpose of this research was to investigate the physiological and molecular basis of B. japonicus resistance to flucarbazone-sodium. Dose-response analysis demonstrated that, compared with the susceptible B. japonicus (S) population, the resistant (R) population exhibited a 120-fold increase in flucarbazone-sodium resistance. Nucleotide sequence alignment of the ALS gene indicated that the Pro-197-Ser mutation in ALS was associated with resistance to flucarbazone-sodium in the R population. The results of a malathion pretreatment study showed that B. japonicus might also have remarkable cytochrome P450 monooxygenase (P450)-mediated metabolic resistance. This is the first report of a Pro-197-Ser mutation and P450-mediated metabolism conferring resistance to flucarbazone-sodium in B. japonicus.