皮肤感染是人类健康的主要威胁,每10000人年造成500起发病率。在糖尿病患者中，特别是,皮肤感染通常伴随着缓慢的愈合过程，截肢,甚至死亡。皮肤感染菌株的及时诊断和现场治疗对人类健康和安全至关重要。在这里，开发了双层“测试-治疗”垫，用于视觉监测和选择性治疗药物敏感（DS）/耐药（DR）细菌感染。内层(使用角叉菜胶水凝胶作为支架)装载有细菌指示剂和酸响应性药物(Fe-羧苄青霉素框架),用于感染检测和DS细菌灭活。外层是机械发光材料(ML，CaZnOS：Mn2）和可见光响应性光催化剂（Pt@TiO2）结合了弹性聚二甲基硅氧烷（PDMS）。根据比色传感结果（DS细菌感染为黄色，DR细菌感染为红色），指导合适的抗菌策略，然后进行。由双垫层提供的两种可用的杀菌途径反映了该优点。通过在机械力作用下由Pt@TiO2和ML的组合原位产生的活性氧（ROS），实现了对DR细菌的可控有效杀灭，避免物理光源，减轻ROS在生物医学治疗中的脱靶副作用。作为一个概念证明,“测试-治疗”垫用作可穿戴的伤口敷料，用于在体外和体内感知和选择性处理DS/DR细菌感染。这种多功能设计有效减少抗生素滥用，加速伤口愈合，在即时诊断和治疗中提供创新和有前途的创可贴策略。
Skin infections are major threats to human health, causing ∼500 incidences per 10 000 person-year. In patients with diabetes mellitus, particularly, skin infections are often accompanied by a slow healing process, amputation, and even death. Timely diagnosis of skin infection strains and on-site therapy are vital in human health and safety. Herein, a double-layered \"test-to-treat\" pad is developed for the visual monitoring and selective treatment of drug-sensitive (DS)/drug-resistant (DR) bacterial infections. The inner layer (using carrageenan hydrogel as a scaffold) is loaded with bacteria indicators and an acid-responsive drug (Fe-carbenicillin frameworks) for infection detection and DS bacteria inactivation. The outer layer is a mechanoluminescence material (ML, CaZnOS:Mn2+) and visible-light responsive photocatalyst (Pt@TiO2) incorporated elastic polydimethylsiloxane (PDMS). On the basis of the colorimetric sensing result (yellow for DS-bacterial infection and red for DR-bacterial infection), a suitable antibacterial strategy is guided and then performed. Two available bactericidal routes provided by double pad layers reflect the advantage. The controllable and effective killing of DR bacteria is realized by in situ generated reactive oxygen species (ROSs) from the combination of Pt@TiO2 and ML under mechanical force, avoiding physical light sources and alleviating off-target side effects of ROS in biomedical therapy. As a proof-of-concept, the \"test-to-treat\" pad is applied as a wearable wound dressing for sensing and selectively dealing with DS/DR bacterial infections in vitro and in vivo. This multifunctional design effectively reduces antibiotic abuse and accelerates wound healing, providing an innovative and promising Band-Aid strategy in point-of-care diagnosis and therapy.
