大骨缺损,通常是由创伤和疾病引起的,提出了重大的临床挑战。与天然ECM的形态和结构非常相似的电纺纤维支架对骨组织工程非常感兴趣。然而,传统的静电纺纤维支架有一定的局限性,包括缺乏相互连接的大孔和表现为2D支架。为了应对这些挑战,通过创新和方便的方法开发了海绵状电纺聚(L-乳酸)(PLLA)/聚己内酯(PCL)纤维支架(即,静电纺丝,均质化,progen浸出和成型)。所得支架表现出高度多孔的结构(总孔隙率=85.9%),相互连接,规则大孔,模仿天然细胞外基质。此外,生物活性玻璃(BG)颗粒的掺入改善了亲水性(水接触角=79.7°)和生物相容性,并促进了成骨细胞的生长。体外10天实验表明,支架导致高细胞活力。增殖率的增量在第7天为195.4%,在第10天为281.6%。更重要的是,Saos-2细胞可以生长,增殖,渗透到支架中。因此,这种带有BG海绵的3DPLLA/PCL在组织工程应用中对骨缺损修复具有广阔的前景。
Large bone defects, often resulting from trauma and disease, present significant clinical challenges. Electrospun fibrous scaffolds closely resembling the morphology and structure of natural ECM are highly interested in bone tissue engineering. However, the traditional electrospun fibrous scaffold has some limitations, including lacking interconnected macropores and behaving as a 2D scaffold. To address these challenges, a sponge-like electrospun poly(L-lactic acid) (PLLA)/
polycaprolactone (PCL) fibrous scaffold has been developed by an innovative and convenient method (i.e., electrospinning, homogenization, progen leaching and shaping). The resulting scaffold exhibited a highly porous structure (overall porosity = 85.9 %) with interconnected, regular macropores, mimicking the natural extracellular matrix. Moreover, the incorporation of bioactive glass (BG) particles improved the hydrophilicity (water contact angle = 79.7°) and biocompatibility and promoted osteoblast cell growth. In-vitro 10-day experiment revealed that the scaffolds led to high cell viability. The increment of the proliferation rates was 195.4 % at day 7 and 281.6 % at day 10. More importantly, Saos-2 cells could grow, proliferate, and infiltrate into the scaffold. Therefore, this 3D PLLA/PCL with BG sponge holds great promise for bone defect repair in tissue engineering applications.