铜和硫化氢污染造成的污染对环境和生物都有严重的不利影响。实时,Cu2+和S2-的快速准确监测面临严峻挑战。在这项研究中,我们设计了一种新型的生物传感器,并通过模拟牛血清白蛋白上主要的Cu(II)结合位点的结构来合成它。作为一种基于肽的传感器,FGGH(FITC-Gly-Gly-His-NH2)可以通过荧光和比色法进行Cu2和S2-的顺序检测。高水溶性和选择性使其适用于以高灵敏度监测环境水样中的Cu2和S2-;其检测限(LOD)对于Cu2低至1.42nM,对于S2-低至22.2nM。该探针的基于纸张的传感平台由于其快速响应和可回收的检测特性而被发现是用于Cu2和S2-的实时定量分析的现场可视化的有前途的工具。此外,FGGH成功地用于在活细胞和斑马鱼模型中成像Cu2和S2-,具有足够的荧光稳定性和低细胞毒性,提供了Cu2和S2-之间相互作用对生物体氧化还原稳态影响的第一个视觉证据。
Pollution caused by Copper and hydrogen sulfide pollution has severe adverse effects on the environment and organisms. Real-time, fast and accurate monitoring of Cu2+ and S2- faces serious challenges. In this study, we designed a novel biosensor and synthesized it by mimicking the structure of the main Cu(II)-binding site on bovine serum albumin. As a peptide-based sensor, FGGH (FITC-Gly-Gly-His-NH2) can perform the sequential detection of Cu2+ and S2- by fluorescence and colorimetry. The high water solubility and selectivity make it suitable for monitoring Cu2+ and S2- in environmental water samples with high sensitivity; its limit of detection (LOD) is as low as 1.42 nM for Cu2+ and 22.2 nM for S2-. The paper-based sensing platform of this probe was found to be a promising tool for the on-site visualization of real-time quantitative analysis of Cu2+ and S2- due to its rapid response and recyclable detection characteristics. Additionally, FGGH was successfully used to image Cu2+ and S2- in living cells and zebrafish models with adequate fluorescence stability and low cytotoxicity, providing the first visual evidence of the effect of the interactions between Cu2+ and S2- on the redox homeostasis of organisms.