How to distinguish and quantify past human impacts on vegetation is a significant challenge in paleoecology. Here, we propose a novel method, the error inflection point-discriminant technique. It finds out the inflection points (IPs) of the regression errors of pollen-climate transfer functions using modern pollen spectra from vegetation with different values of the Human Influence Index (HII), which represent the HII threshold values of native/secondary and secondary/artificial vegetation systems. Our results show that the HII value at the native/secondary vegetation IPs is approximately 22 and globally uniform, whereas it varies regionally for the secondary/artificial vegetation IPs. In a case study of the Liangzhu archaeological site in the lower Yangtze River, discriminant functions for pollen spectra from three vegetation types and pollen-climate transfer functions of the native vegetation were established to reconstruct paleovegetation and paleoclimate over the past 6,600 years. Our study demonstrates this method\'s feasibility for quantitatively distinguishing human impacts on paleovegetation and assessing quantitative paleoclimate reconstructions using pollen data.

Phytoliths are a reliable paleovegetation proxy and have made an important contribution to paleoclimatic studies. However, little is known about the depositional processes affecting soil phytoliths, which limits their use for paleoclimate and paleovegetation reconstructions. Here, we present the results of a study of the vertical translocation characteristics of phytoliths in 40 natural soil profiles in Northeast China. The results show that phytolith concentration decreases within the humic horizon of the soil profiles and that ∼22% of the phytoliths are translocated below the surface of the studied soils. In addition, we find that the translocation rate of phytoliths varies markedly with phytolith type and that phytolith size and aspect ratio also have a significant effect. Phytoliths with length >30 μm and with aspect ratio >2 and those with length <20 μm and aspect ratio <2 are preferentially translocated compared to those with length >25 μm and aspect ratio <2. Our results demonstrate that differential translocation of phytoliths within soil profiles should be considered when using soil phytoliths for paleoclimate and paleovegetation reconstruction.