背景：儿科发育过程中肌纤维不同形态参数的标准值，即从0到18岁，目前不可用。它们对于准确评估病理变化具有重要意义，可用作临床试验中评估治疗反应的参考生物标志物。或运动或衰老中的生理调整。
方法：数据来自482张图像，共33.094纤维来自83例患者三角肌活检的10μm速冻肌肉横截面，0-18岁,无神经肌肉病理学染色ATPase9.4。采用来自“CARPACCIO”的专利图像分析算法采集和分析数据。云\"。提取或计算了几个参数，包括横截面积(CSA)，纤维类型,循环性,以及Feret的最小直径（MinFeret）。
结果：这项研究说明了在儿科发育过程中肌肉形态的定量参数变化以及青春期前后发生的关键变化。仅纤维尺寸参数(MinFeret，CSA)取决于性别，只有在青春期之后.所有其他参数对于女性和男性以类似的方式变化。从出生到10岁，1型纤维的比例基本上是恒定的，到18岁时下降到约40％。圆形随着年龄的增长而减少，两种纤维类型都在10岁后达到平稳状态。
结论：已经生成了该年龄范围内的肌纤维类型的规范值和参考图表，以比较病理实验室从事神经肌肉疾病工作的患者的数据。
背景：BPI法国，Pulsalys,Myologie研究所协会，法国国家研究机构（ANR）LABEXCORTEX的里昂大学。
BACKGROUND: Normative values for different morphometric parameters of muscle fibres during paediatric development, i.e. from 0 to 18 years, are currently unavailable. They would be of major importance to accurately evaluate pathological changes and could be used as reference biomarkers for evaluating treatment response in clinical trials, or physiological adjustments in sports or ageing.
METHODS: Data were derived from 482 images with a total of 33 094 fibres from 10 μm cross-sections of snap-frozen muscle from 83 deltoid muscle biopsies from patients, 0-18 years, without neuromuscular pathology stained with ATPase 9.4. Data was acquired and analysed with patented image analysis algorithms from \"CARPACCIO.cloud\". Several parameters were extracted or calculated, including cross-sectional area (CSA), fibre type, circularity, as well as the Minimum diameter of Feret (MinFeret).
RESULTS: This study illustrates changes in quantitative parameters for muscle morphology over the course of paediatric development and the pivotal changes occurring around puberty. Only fibre size parameters (MinFeret, CSA) are dependent on gender, and only after puberty. All other parameters vary in a similar manner for females and males. The proportion of type 1 fibres is essentially constant from birth to age 10, decreasing to ≈40% by age 18. Circularity decreases with age, to plateau after age 10 for both fibre types.
CONCLUSIONS: Normative values and reference charts for muscle fibre types in this age range have been generated to allow comparison of data from patients in pathology laboratories working on neuromuscular diseases.
BACKGROUND: BPI FRANCE, PULSALYS, Association de l\'Institut de Myologie, French National Research Agency (ANR), LABEX CORTEX of Université de Lyon.
