苦参是豆科中苦参属的灌木,具有抗炎和缓解疼痛的作用。虽然种植,化学化妆品,并已报道了山雀的药用特性,决定其脱水和种子冷冻保存耐受性的生理机制仍不清楚。在这项研究中,我们调查了形态学,生理,生物化学,通过细胞微观结构的观察,对脱水和冷冻保存技术的山地根草种子的蛋白质表达特性,抗氧化酶活性的测定,和基于iTRAQ的蛋白质组学分析,分别。研讨成果注解,种子对脱水具有必定水平的耐性。脱水2小时后观察到83.0%的最高发芽率(10.1%的含水量),这被确定为冷冻保存的最佳时间点。然而,当含水量达到5.4%时,发芽率降低到只有30.5%,表明山葵种子表现出中间贮藏行为。进一步调查发现,在种子脱水和冷冻保存处理期间,脂质体逐渐高度融合,而ROS清除和应激防御的活性显著增强。脱水期间,种子组织在内质网和抗氧化系统的蛋白质加工基础上形成了抗逆保护机制,这与脱水耐受性有关。此外,仅鉴定出三种差异表达的LEA蛋白,据推测,细胞内代谢的加强以及缺乏特定的LEA和脱水蛋白可能是过度脱水和冷冻保存后发芽率降低的关键因素。
Sophora tonkinensi is a shrub of the genus Sophora in the family Fabaceae with anti-inflammatory and pain-relieving effects. While the cultivation, chemical makeup, and medicinal properties of S. tonkinensis have been reported, the physiological mechanisms governing its dehydration and cryopreservation tolerance of seeds remain unclear. In this study, we investigated the morphological, physiological, biochemical, and protein expression characteristics of S. tonkinensis seeds subjected to dehydration and cryopreservation techniques via the observation of cell microstructure, determination of antioxidant enzyme activity, and iTRAQ-based proteomic analysis, respectively. The results of the study demonstrated that the seeds possessed a certain level of tolerance to dehydration. The highest germination percentage of 83.0% was observed after 2 h of dehydration (10.1% water content), which was identified as the optimal time point for cryopreservation. However, the germination percentage was reduced to only 30.5% when the water content reached 5.4%, indicating that S. tonkinensis seeds exhibit intermediate storage behavior. Further investigation revealed that during seed dehydration and cryopreservation treatment, liposomes were gradually and highly fused, whereas the activities of ROS scavenging and stress defense were significantly enhanced. During dehydration, the seed tissues formed a protective mechanism of stress resistance based on protein processing in the endoplasmic reticulum and antioxidant system, which was related to the dehydration tolerance. Moreover, only three differentially expressed LEA proteins were identified, and it is speculated that the strengthening of intracellular metabolism and the absence of specific LEA and dehydrins could be crucial factors for the reduced germination percentage after excessive dehydration and cryopreservation.