植物多酚补充可以改善水产养殖系统中的鱼类健康。评估植物多酚在水产养殖中的潜在效益和作用机制。给鱼喂食基础饲料(CON)或补充500mg/kg姜黄素(CUR)的基础饲料,低聚原花色素(OPC),绿原酸(CGA),或白藜芦醇(RES)。经过8周的喂养实验,血液样本用于分析生化指标的浓度。收集肠道样本以评估微生物群,短链脂肪酸(SCFA)水平,和基因表达。结果表明,多酚给药降低了血清葡萄糖和胰岛素。OPC和CGA增强了溶菌酶活性,超氧化物歧化酶活性被CUR增加,OPC,和CGA。RES组的肠道微生物结构与CON的肠道微生物结构分离,拟杆菌属被鉴定为CUR中的潜在生物标志物,CGA,和RES组。总肠道SCFA在CUR中增加,CGA,和RES组。在拟杆菌和SCFA之间观察到强相关性。总之,膳食多酚具有明显的抗炎作用,抗氧化剂,和可能与其微生物群调节作用密切相关的抗高血糖活性。
Plant polyphenol supplementation may improve fish health in aquaculture systems. To assess the potential benefits and function mechanism of plant polyphenols in aquaculture, fish were fed either basal feed (CON) or the basal feed supplemented with 500 mg/kg of curcumin (CUR), oligomeric proanthocyanidins (OPC), chlorogenic acid (CGA), or resveratrol (RES). After an 8-week feeding experiment, blood samples were used to analyze the concentrations of biochemical indices. Gut samples were collected to evaluate microbiota, short chain fatty acid (SCFA) levels, and gene expression. The results indicated that polyphenol administration reduced serum glucose and insulin. Lysozyme activity was enhanced by OPC and CGA, and superoxide dismutase activity was increased by CUR, OPC, and CGA. The gut microbial structure of the RES group was segregated from that of the CON, and the genus Bacteroides was identified as a potential biomarker in the CUR, CGA, and RES groups. Total gut SCFA increased in the CUR, CGA, and RES groups. A strong correlation was observed between Bacteroides and SCFA. In conclusion, dietary polyphenols have distinct anti-inflammatory, anti-oxidant, and anti-hyperglycemic activities that may be closely associated with their microbiota-modulation effects.