羊肉桂(绵羊ked)是绵羊中常见的体外寄生虫之一。除了通过咬和吸血对宿主造成直接伤害之外,绵羊Ked是蠕虫的潜在载体,原生动物,细菌,和病毒。选择来自西藏三个地区的绵羊M.ovinus样品进行DNA提取。扩增了16SrDNAV3-V4高变区,基因组DNA片段化后,构建IlluminaHiseq文库。在IlluminaNovaseq6000平台上分别进行16SrRNA测序和病毒宏基因组学测序,并采用分子生物学软件和平台分析测序数据。IlluminaPE250测序结果表明,西藏产卵分枝杆菌中的优势菌门,中国是变形杆菌,其中注释了29个细菌属。优势细菌属为巴尔通体,Wolbachia,和阿瑟诺克斯;夜蛾,Wolbachiaspp.,和Senophonusspp。是来自西藏的M.ovinus中的优势细菌,中国。我们还检测到了Kluyveraintermedia,马氏棒杆菌DSM45190,盘根微生物,和红球菌,其中中间Kluyvera的相对丰度很高。IlluminaHiseq测序结果表明,在西藏的M.ovinus中检测到4种病毒,中国,3个样本被注释到29个家庭中,30个家庭,和28个病毒家族,分别。与脊椎动物和昆虫相关的病毒家族主要包括拟南芥科,马赛莱维科,痘病毒科,子囊病毒科,轮状病毒科,杆状病毒科,Hytrosaviridae,Nutriridae,多义科,Adomaviridae,Asfarviridae,Hepevirridae,疱疹病毒科,和逆转录病毒科;在物种层面,Tupanvirus_soda_lake的相对丰度,Klosneuvirus_KNV1和Indivirus_ILV1较高。检测到非洲猪瘟病毒和许多痘病毒,尽管它们的相对丰度很低。西藏产卵分枝杆菌优势菌门,中国是变形杆菌,优势细菌属是巴尔通体,Wolbachia,和砷,其中29个注释细菌属中的23个首先在M.ovinus中报道。克鲁维拉中间媒体,马氏棒杆菌DSM45190,盘根微生物,并首次检出红斑红球菌。在这项研究中检测到的所有DNA病毒均首次在M.ovinus中报道。
Melophagus ovinus (sheep ked) is one of the common ectoparasites in sheep. In addition to causing direct damage to the host through biting and sucking blood, sheep ked is a potential vector of helminths, protozoa, bacteria, and viruses. Sheep M. ovinus samples from three regions in Tibet were selected for DNA extraction. The 16S rDNA V3-V4 hypervariable region was amplified, after genomic DNA fragmentation, Illumina Hiseq libraries were constructed. The 16S rRNA sequencing and viral metagenomics sequencing were separately conducted on the Illumina Novaseq 6000 platform and molecular biology software and platforms were employed to analyze the sequencing data. Illumina PE250 sequencing results demonstrated that the dominant bacteria phylum in M. ovinus from Tibet,
China was Proteobacteria, where 29 bacteria genera were annotated. The dominant bacterial genera were Bartonella, Wolbachia, and Arsenophonus; Bartonella chomelii, Wolbachia spp., and Arsenophonus spp. were the dominant bacterial species in M. ovinus from Tibet,
China. We also detected Kluyvera intermedia, Corynebacterium maris DSM 45190, Planomicrobium okeanokoites, and Rhodococcus erythropolis, of which the relative abundance of Kluyvera intermedia was high. Illumina Hiseq sequencing results demonstrated that 4 virus orders were detected in M. ovinus from Tibet,
China, and 3 samples were annotated into 29 families, 30 families, and 28 families of viruses, respectively. Virus families related to vertebrates and insects mainly included Mimiviridae, Marseilleviridae, Poxviridae, Ascoviridae, Iridoviridae, Baculoviridae, Hytrosaviridae, Nudiviridae, Polydnaviridae, Adomaviridae, Asfarviridae, Hepeviridae, Herpesviridae, and Retroviridae; at the species level, the relative abundance of Tupanvirus_soda_lake, Klosneuvirus_KNV1, and Indivirus_ILV1 was higher. African swine fever virus and many poxviruses from the family Poxviridae were detected, albeit their relative abundance was low. The dominant bacterial phylum of M. ovinus from Tibet,
China was Proteobacteria, and the dominant bacterial genera were Bartonella, Wolbachia, and Arsenophonus, where 23 out of 29 annotated bacteria genera were first reported in M. ovinus. Kluyvera intermedia, Corynebacterium maris DSM 45190, Planomicrobium okeanokoites, and Rhodococcus erythropolis were detected for the first time. All DNA viruses detected in this study have been reported in M. ovinus for the first time.