非洲猪瘟病毒(ASFV)感染导致ASF,一种高度传染性和致命的疾病,对猪的生产构成严重威胁。为了深入了解主机对ASFV的反应,我们分别产生了表达病毒膜蛋白p54,p17和pB117L的重组腺病毒Ad5,并感染了肺泡细胞系,3D4/21,与这些重组病毒。使用无标记定量蛋白质组分析方法分析细胞裂解物。共鉴定出2,158种差异表达蛋白(DEP),其中817、466和875蛋白来自Ad5-p54-,Ad5-p17-,Ad5-pB117L感染的3D4/21细胞,分别。基因本体论(GO)分类和京都基因和基因组百科全书(KEGG)途径富集分析揭示了蛋白质相互作用网络的独特但互连的模式。具体来说,Ad5-p54感染丰富了主要参与代谢途径的DEP,内吞作用,附着者接合处,和SNARE在囊泡运输中的相互作用。Ad5-p17感染丰富了DEP的内吞作用,泛素介导的蛋白水解,N-聚糖生物合成,和细胞凋亡,而Ad5-pB117L感染在代谢途径中丰富了DEP,内吞作用,氧化磷酸化,和病灶粘连。总之,这些结果提供了对三种ASFV膜蛋白的细胞反应的全面蛋白质组学分析,从而提高我们对ASFV发病机制的认识。
African swine fever virus (ASFV) infection causes African swine fever (ASF), a highly contagious and fatal disease that poses severe threat to swine production. To gain insights into the host responses to ASFV, we generated recombinant adenovirus Ad5 expressing viral membrane proteins p54, p17, and pB117L individually and infected an alveolar cell line, 3D4/21, with these recombinant viruses. Then, the cell lysates were analyzed using label-free quantification proteomic analysis method. A total of 2158 differentially expressed proteins (DEPs) were identified, of which 817, 466, and 875 proteins were from Ad5-p54-, Ad5-p17-, Ad5-pB117L-infected 3D4/21 cells, respectively. Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed distinct yet interconnecting patterns of protein interaction networks. Specifically, the Ad5-p54 virus infection enriched the DEPs primarily involved in the metabolic pathways, endocytosis, adherens junction, and SNARE interactions in vesicular transport. The Ad5-p17 virus infection enriched the DEPs in endocytosis, ubiquitin-mediated proteolysis, N-Glycan biosynthesis, and apoptosis, while the Ad5-pB117L virus infection enriched the DEPs in metabolic pathways, endocytosis, oxidative phosphorylation, and focal adhesion. In summary, these results provide a comprehensive proteinomics analysis of the cellular responses to three ASFV membrane proteins, thus facilitating our understanding of ASFV pathogenesis.