■色素沉着过度,一种常见的皮肤状况,以黑色素产生过多为特征,目前有效的治疗方案有限。
■本研究探讨了桃红四五汤对色素沉着的影响,并阐明了其潜在机制。
■我们采用了网络药理学,孟德尔随机化,和分子对接,以确定THSWD的枢纽靶标和防止色素沉着过度的机制。细胞计数试剂盒-8(CCK-8)测定确定了PIG1细胞的合适的THSWD处理浓度。这些细胞暴露于分级浓度的含THSWD的血清(2.5%,5%,10%,15%,20%,30%,40%,和50%),并用α-MSH(100nM)处理以诱导体外色素沉着过度模型。评估包括黑色素含量,酪氨酸酶活性,和西方印迹。
■ALB,IL6和MAPK3成为主要目标,而槲皮素,芹菜素,木犀草素是核心活性成分。CCK-8试验表明浓度在2.5%和20%之间适合PIG1细胞,50%的细胞毒性浓度(CC50)为32.14%。THSWD处理显著降低α-MSH诱导的PIG1细胞的黑色素含量和酪氨酸酶活性,同时下调MC1R和MITF表达。THSWD增加了模型细胞中ALB和p-MAPK3/MAPK3的水平,并降低了IL6的表达。
■THSWD通过靶向ALB缓解色素沉着过度,IL6和MAPK3。这项研究为THSWD作为一种新的色素沉着治疗方法的临床应用铺平了道路,并提供了新的靶向治疗策略。
UNASSIGNED: Hyperpigmentation, a common skin condition marked by excessive melanin production, currently has limited effective treatment options.
UNASSIGNED: This study explores the effects of Tao-Hong-Si-Wu decoction (THSWD) on hyperpigmentation and to elucidate the underlying mechanisms.
UNASSIGNED: We employed network pharmacology, Mendelian randomization, and molecular docking to identify THSWD\'s hub targets and mechanisms against hyperpigmentation. The Cell Counting Kit-8 (CCK-8) assay determined suitable THSWD treatment concentrations for PIG1 cells. These cells were exposed to graded concentrations of THSWD-containing serum (2.5%, 5%, 10%, 15%, 20%, 30%, 40%, and 50%) and treated with α-MSH (100 nM) to induce an in vitro hyperpigmentation model. Assessments included melanin content, tyrosinase activity, and Western blotting.
UNASSIGNED: ALB, IL6, and MAPK3 emerged as primary targets, while quercetin, apigenin, and luteolin were the core active ingredients. The CCK-8 assay indicated that concentrations between 2.5% and 20% were suitable for PIG1 cells, with a 50% cytotoxicity concentration (CC50) of 32.14%. THSWD treatment significantly reduced melanin content and tyrosinase activity in α-MSH-induced PIG1 cells, along with downregulating MC1R and MITF expression. THSWD increased ALB and p-MAPK3/MAPK3 levels and decreased IL6 expression in the model cells.
UNASSIGNED: THSWD mitigates hyperpigmentation by targeting ALB, IL6, and MAPK3. This study paves the way for clinical applications of THSWD as a novel treatment for hyperpigmentation and offers new targeted therapeutic strategies.