骨髓间充质干细胞(BMSCs)在再生医学中有着广泛的应用。然而,目前尚不清楚BMSCs移植是否能改善X连锁肌营养不良小鼠(mdx)的心功能以及如何检测。我们旨在研究斑点追踪超声心动图(STE)在检测BMSCs移植mdx与未治疗mdx相比的心功能中的作用。
实验小鼠分为BMSCs移植mdx,未经处理的MDX,和对照小鼠组(每组n=6)。在20周龄时通过尾静脉注射将BMSC移植到mdx的亚组中。四周后,对3组小鼠的心功能参数进行超声心动图分析。然后,所有的老鼠都被处死了,收集心脏组织并通过免疫荧光进行分析。还分析了血清生化参数以确定BMSCs移植的有益效果。
三组小鼠BMSCs移植后,传统超声心动图参数未显示统计学上的显着差异。与对照组相比,mdx在长轴和短轴左心室图像中均显示出明显较低的左心室(LV)STE参数(P&lt;0.05)。然而,BMSCs移植的mdx在几个STE参数中显示出改善,包括一些STE参数的显着增加(P&lt;0.05)。心肌组织的免疫荧光染色显示mdx与对照小鼠之间的统计学差异(P&lt;0.05),mdx移植的骨髓间充质干细胞与未处理的mdx相比有显著改善(P<0.05)。
这项研究表明,通过STE可以准确检测到mdx中LV收缩和舒张功能的早期降低。此外,我们的研究表明,在mdx中,BMSCs的移植显着改善了心肌功能。
Bone marrow mesenchymal stem cells (BMSCs) are commonly used in regenerative medicine. However, it is not clear whether transplantation of BMSCs can improve cardiac function of the X-Linked Muscular Dystrophy Mice (
mdx) and how to detect it. We aimed to investigate the role of speckle tracking echocardiography (STE) in detecting cardiac function of the BMSCs-transplanted mdx in comparison with the untreated
mdx.
The experimental mice were divided into the BMSCs-transplanted
mdx, untreated
mdx, and control mice groups (n = 6 per group). The BMSCs were transplanted via tail vein injections into a subset of
mdx at 20 weeks of age. After four weeks, the cardiac functional parameters of all the mice in the 3 groups were analyzed by echocardiography. Then, all the mice were sacrificed, and the cardiac tissues were harvested and analyzed by immunofluorescence. The serum biochemical parameters were also analyzed to determine the beneficial effects of BMSCs transplantation.
Traditional echocardiography parameters did not show statistically significant differences after BMSCs transplantation for the three groups of mice. In comparison with the control group, mdx showed significantly lower left ventricular (LV) STE parameters in both the long-axis and short-axis LV images (P < 0.05). However, BMSCs-transplanted mdx showed improvements in several STE parameters including significant increases in a few STE parameters (P < 0.05). Immunofluorescence staining of the myocardium tissues showed statistically significant differences between the mdx and the control mice (P < 0.05), and the mdx transplanted with BMSCs demonstrated significantly improvement compared with the untreated
mdx (P < 0.05).
This study demonstrated that the early reduction in the LV systolic and diastolic function in the mdx were accurately detected by STE. Furthermore, our study demonstrated that the transplantation of BMSCs significantly improved myocardial function in the mdx.