伤口感染容易受到感染性病原体的攻击,包括使常规抗菌药物无效的多药耐药细菌。最近,已提出将溶素用作常规抗微生物剂的替代品,以应对多药耐药性病原体的威胁。溶素与将覆盖伤口的材料的偶联可以证明在保护和治疗伤口感染方面都是有益的。因此,在这项研究中,革兰氏阴性溶素,LysP53与热敏水凝胶偶联,测试了泊洛沙姆P407及其治疗伤口感染的功效。体外,在泊洛沙姆中加入LysP53并不影响其热敏特性,也不影响水凝胶结构。此外,溶素水凝胶可以水解肽聚糖,证明它可能具有杀菌活性。高达10.4%的LysP53在24小时内逐渐从水凝胶中释放,这导致鲍曼不动杆菌的稳定期减少4-log。最后,发现溶素水凝胶是安全的,在细胞中没有观察到细胞毒性作用。离体,LysP53水凝胶在猪皮脱色模型上可以抑制细菌生长,与未治疗组相比,差异为3-log。总的来说,我们的研究结果表明,溶素水凝胶可能为治疗耐药细菌引起的伤口感染提供一种新的解决方案.
Wound infections are prone to attacks from infectious pathogens, including multidrug resistant bacteria that render conventional antimicrobials ineffective. Recently, lysins have been proposed as alternatives to conventional antimicrobials to tackle the menace of multidrug resistance pathogens. The coupling of lysins with a material that will cover the wound may prove beneficial in both protecting and treating wound infections. Hence, in this study, a Gram-negative
lysin, LysP53, was coupled with a thermosensitive hydrogel, poloxamer P407, and its efficacy to treat wound infection was tested. In vitro, the addition of LysP53 to the poloxamer did not affect its thermosensitive characteristics, nor did it affect the hydrogel structure. Moreover, the
lysin hydrogel could hydrolyze the peptidoglycan, demonstrating that it may have bactericidal activity. Up to 10.4% of LysP53 was released from the hydrogel gradually within 24 h, which led to a 4-log reduction of stationary phase Acinetobacter baumannii. Lastly, the
lysin hydrogel was found safe with no cytotoxic effects observed in cells. Ex vivo, LysP53 hydrogel could inhibit bacterial growth on a pig skin decolonization model, with 3-log differences compared to non-treated groups. Overall, our results suggest that
lysin-loaded hydrogels may provide a novel solution to treat wound infections caused by resistant bacteria.