■参附(SF)注射液,一种传统的中药,改善心源性休克和感染性休克的微循环。本研究旨在探讨SF注射液在严重失血性休克(SHS)和复苏后肠缺血再灌注(I/R)损伤中的治疗潜力。此外,我们还研究了最优adm?注入时间。
■24只雄性SD大鼠随机分为4组:假手术组(假手术组,n=6),对照组(n=6),SF注射组(SF,n=6),和延迟参附注射液给药组(SF延迟,n=6)。在SHS和复苏模型中,大鼠在1小时内抽血至平均动脉压(MAP)为40±5mmHg,然后维持40分钟;HR,地图被记录下来,微循环指数[DeBacker评分,灌注小血管密度(PSVD),总血管密度(TVD),微循环流量指数评分(MFI),流量异质性指数(HI)]进行了分析。检测血气指标,白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α),二胺氧化酶(DAO),通过ELISA测量丙二醛(MDA);通过Western印迹测量ZO-1和claudin-1。此外,还进行了肠粘膜组织的苏木精-伊红(HE)和高碘酸希夫(PAS)染色病理切片。
■SF注入增加了MAP,缓解与低灌注相关的代谢性酸中毒程度,改善了I/R损伤后肠微循环密度和灌注质量。DAO的表达式,肠组织中的MDA,与对照组相比,SF注射组的血浆IL-6,TNF-α明显降低。在SF注射组中ZO-1和claudin-1的浓度也较高。此外,HE和PAS染色结果还表明,SF注射液可减轻粘膜损伤并维持结构。在SF延迟组中,肠组织损伤程度介于对照组和SF注射组之间。
■SF注射液保护肠道免受SHS和复苏引起的I/R损伤,其机制可能是通过改善肠道微循环,减少炎症因子的过度释放,增加肠粘膜通透性。此外,如果在初始复苏阶段给药,则保护作用更为明显。
UNASSIGNED: Shenfu (SF) injection, a traditional Chinese medication, would improve microcirculation in cardiogenic shock and infectious shock. This study was aimed to explore the therapeutic potential of the SF injection in gut ischemia-reperfusion (I/R) injury after severe hemorrhagic shock (SHS) and resuscitation. Furthermore, we also investigated the optimal adm? inistration timing.
UNASSIGNED: Twenty-four male SD rats were randomly divided into four groups: Sham group (sham, n = 6), Control group (n = 6), SF injection group (SF, n = 6), and Delayed Shenfu injection administration group (SF-delay, n = 6). In SHS and resuscitation model, rats were induced by blood draw to a mean arterial pressure (MAP) of 40 ± 5 mmHg within 1 h and then maintained for 40 min; HR, MAP \'were recorded, microcirculation index [De Backer score, perfused small vessel density (PSVD), total vessel density (TVD), microcirculation flow index score (MFI), flow heterogeneity index (HI)] were analyzed. The blood gas index was detected, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), diamine oxidase (DAO), malondialdehyde (MDA) were measured by ELISA; ZO-1, and claudin-1 were measured by Western blotting. In addition, hematoxylin-eosin (HE) and periodic acid schiff (PAS) staining pathological sections of the intestinal mucosal tissues were also performed.
UNASSIGNED: SF injection increased the MAP, relieved the metabolic acidosis degree associated with the hypoperfusion, and improved the intestinal microcirculatory density and perfusion quality after I/R injury. The expression of DAO, MDA in intestinal tissue, and plasma IL-6, TNF-α significantly decreased in the SF injection group compared to the control group. The concentration of ZO-1 and claudin-1 is also higher in the SF injection group. In addition, the HE and PAS staining results also showed that SF injection could decrease mucosal damage and maintain the structure. In the SF-delay group, the degree of intestinal tissue damage was intermediate between that of the control group and SF injection group.
UNASSIGNED: SF injection protect the intestine from I/R injury induced by SHS and resuscitation, the mechanism of which might be through improving intestinal microcirculation, reducing the excessive release of inflammatory factors and increasing intestinal mucosal permeability. Furthermore, the protection effect is more pronounced if administration during the initial resuscitation phase.