Anaerobic digestion of lignocelluloses for biogas production is greatly restricted by the poor biomass degradability. Herein, a novel approach is suggested to enhance the energy recovery from rice straw through a two-step conversion using eggshell-based catalytic pyrolysis followed by biochar-based anaerobic co-digestion. Pyrolysis with eggshell significantly enhanced the crude bio-oil yield by 4.6 %. Anaerobic digestion of rice straw using 4 g L-1 of rice straw biochar (RB) showed the highest recorded biogas yield of 503.7 L kg-1 VS, with 268.6 L kg-1 VS biomethane yield. However, 4 g L-1 of calcium-enriched eggshell rice straw biochar (ERB) enhanced the biomethane yield to 281.8 L kg-1 VS, which represented 95.6 % higher than the control. It was attributed to enhancement of biomethanation, which resulted in 74.5 % maximum recorded biomethane content at the 7th day of anaerobic digestion. Microbial analysis confirmed that Methanosarciniales was the most dominant Archael group in the control (14.84 %), which increased sharply to 73.91 % and 91.66 % after addition of 4 g L-1 RB and ERB, respectively. The suggested route enhanced the energy recovery in the form of bio-oil and biomethane by 41.6 %.

Parametric thinking has found wide acceptance in both the building industry and environmental sciences. In the context of environmental urban morphology however, parametric thinking has been neglected. This paper critically assesses environmental optimization with a focus on parametric urbanism. The analysis addresses two research questions: \"Can parametric thinking and its associated approaches facilitate an environmental urban morphology?\" and \"If yes, can parametric urbanism support environment optimization in complex urban areas?\" Methodologies include a case study in Beijing, qualitative and quantitative analysis, parametric modeling, and environmental simulation. Results indicate that parametric techniques can effectively simulate environmental urban morphology by generating parametric models. These models provide a rational foundation for schematic decision-making about optimizing environments in urban development. Findings include a critique of parametric thinking as applied to city environments and insights about the potential uses of parametric techniques to support quality environmental urban morphology.

The diagnosis of fungal Neglected Tropical Diseases (NTD) is primarily based on initial visual recognition of a suspected case followed by confirmatory laboratory testing, which is often limited to specialized facilities. Although molecular and serodiagnostic tools have advanced, a substantial gap remains between the desirable and the practical in endemic settings. To explore this issue further, we conducted a survey of subject matter experts on the optimal diagnostic methods sufficient to initiate treatment in well-equipped versus basic healthcare settings, as well as optimal sampling methods, for three fungal NTDs: mycetoma, chromoblastomycosis, and sporotrichosis. A survey of 23 centres found consensus on the key role of semi-invasive sampling methods such as biopsy diagnosis as compared with swabs or impression smears, and on the importance of histopathology, direct microscopy, and culture for mycetoma and chromoblastomycosis confirmation in well-equipped laboratories. In basic healthcare settings, direct microscopy combined with clinical signs were reported to be the most useful diagnostic indicators to prompt referral for treatment. The survey identified that the diagnosis of sporotrichosis is the most problematic with poor sensitivity across the most widely available laboratory tests except fungal culture, highlighting the need to improve mycological diagnostic capacity and to develop innovative diagnostic solutions. Fungal microscopy and culture are now recognized as WHO essential diagnostic tests and better training in their application will help improve the situation. For mycetoma and sporotrichosis, in particular, advances in identifying specific marker antigens or genomic sequences may pave the way for new laboratory-based or point-of-care tests, although this is a formidable task given the large number of different organisms that can cause fungal NTDs.

Long-term monitoring, space-for-time substitutions along gradients, and in situ temperature manipulations are common approaches to understand effects of climate change on alpine and arctic plant communities. Although general patterns emerge from studies using different approaches, there are also some inconsistencies. To provide better estimates of plant community responses to future warming across a range of environments, there have been repeated calls for integrating different approaches within single studies. Thus, to examine how different methods in climate change effect studies may ask different questions, we combined three climate warming approaches in a single study in the Hengduan Mountains of southwestern China. We monitored plant communities along an elevation gradient using the space-for-time approach, and conducted warming experiments using open top chambers (OTCs) and plant community transplantation toward warmer climates along the same gradient. Plant species richness and abundances were monitored over 5 years addressing two questions: (1) how do plant communities respond to the different climate warming approaches? (2) how can the combined approaches improve predictions of plant community responses to climate change? The general trend across all three approaches was decreased species richness with climate warming at low elevations. This suggests increased competition from immigrating lowland species, and/or from the species already growing inside the plots, as indicated by increased biomass, vegetation height or proportion of graminoids. At the coldest sites, species richness decreased in OTCs and along the gradient, but increased in the transplants, suggesting that plant communities in colder climates are more open to invasion from lowland species, with slow species loss. This was only detected in the transplants, showing that different approaches, may yield different results. Whereas OTCs may constrain immigration of new species, transplanted communities are rapidly exposed to new neighbors that can easily colonize the small plots. Thus, different approaches ask slightly different questions, in particular regarding indirect climate change effects, such as biotic interactions. To better understand both direct and indirect effects of climate change on plant communities, we need to combine approaches in future studies, and if novel interactions are of particular interest, transplants may be a better approach than OTCs.