背景:血小板减少症是原发性干燥综合征(pSS)的血液学表现之一。本研究的目的是评估抗血小板糖蛋白自身抗体在原发性干燥综合征(pSS)血小板减少症发病机制中的可能作用。
方法:血浆抗糖蛋白Ib水平,在36个无血小板减少症的pSS患者和35个有血小板减少症的pSS患者中,IIIa和IIb/IIIa自身抗体,对36例特发性血小板减少性紫癜(ITP)患者和39例正常对照者进行酶联免疫吸附试验(ELISA)检测。
结果:抗GPIb的水平,GPIIIa,有血小板减少症的pSS中的GPIIb/IIIa自身抗体(A490)显着高于无血小板减少症的pSS(0.813±0.161vs0.688±0.133;0.917±0.094vs0.802±0.070;0.911±0.125vs0.782±0.109)。反GPIb的发生率,GPIIIa,有血小板减少症的pSS中的GPIIb/IIIa自身抗体显着高于无血小板减少症的pSS(25.7%vs0%;65.7%vs11.1%;31.4%vs0%)。在pSS患者中,抗GPIb的血小板计数较低,GPIIIa,GPIIb/IIIa自身抗体阳性患者((25.67±5.5)×10^9/Lvs(116.8±84.52)×10^9/L;29.04±11.33×10^9/Lvs(152.0±75.47)×10^9/L;(31.55±14.0)×10^9/Lvs(118.8±85.24)×10^9/L)。
结论:血浆抗血小板糖蛋白自身抗体水平升高可能在pSS血小板减少症的发病机制中起作用。要点•抗GPIb水平,GPIIIa,血小板减少症pSS中的GPIIb/IIIa自身抗体(A490)增加。•anti-GPIb事件,GPIIIa,血小板减少症的pSS中的GPIIb/IIIa自身抗体增加。•pSS患者,抗GPIb的血小板计数较低,GPIIIa,GPIIb/IIIa自身抗体阳性患者。
BACKGROUND: Thrombocytopenia is one of the primary Sjögren\'s syndrome (pSS) hematological manifestations. The objective of this study was to evaluate the possible roles of antiplatelet glycoprotein autoantibodies in the pathogenesis of thrombocytopenia in primary Sjögren\'s syndrome (pSS).
METHODS: The level of plasma anti-glycoprotein Ib, IIIa and IIb/IIIa autoantibodies in 36 pSS patients without thrombocytopenia and 35 pSS patients with thrombocytopenia, 36
Idiopathic thrombocytopenic purpura (ITP) patients and 39 normal control were measured with enzyme-linked immunosorbent assay (ELISA).
RESULTS: The level of anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies (A490) in the pSS with thrombocytopenia was significantly higher than that of pSS without thrombocytopenia (0.813 ± 0.161 vs 0.688 ± 0.133; 0.917 ± 0.094 vs 0.802 ± 0.070; 0.911 ± 0.125 vs 0.782 ± 0.109). Incidences of the anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies in the pSS with thrombocytopenia was significantly higher than that of pSS without thrombocytopenia (25.7% vs 0%; 65.7% vs 11.1%; 31.4% vs 0%). In patients with pSS, there was a lower platelet count in anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies positive patients ((25.67 ± 5.5) × 10^9/L vs (116.8 ± 84.52) × 10^9/L; 29.04 ± 11.33 × 10^9/L vs (152.0 ± 75.47) × 10^9/L; (31.55 ± 14.0) × 10^9/L vs (118.8 ± 85.24) × 10^9/L).
CONCLUSIONS: Elevated plasma levels of anti-platelet glycoprotein autoantibodies may play a role in the pathogenesis of thrombocytopenia in pSS. Key Points • The level of anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies (A490) in the pSS with thrombocytopenia was increased. • Incidences of the anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies in the pSS with thrombocytopenia was increased. • In patients with pSS, there was a lower platelet count in anti-GPIb, GPIIIa, GPIIb/IIIa autoantibodies positive patients.