急性附睾-睾丸炎的睾丸后果在长期损害中仍未阐明。这可能是男性不育的一个被忽视的因素。在这项研究中,在第1天,第7天和第28天,通过流式细胞术分析了脂多糖(LPS)诱导的小鼠附睾-睾丸炎中睾丸免疫细胞亚群的差异表型.巨噬细胞的数量,中性粒细胞,和骨髓来源的抑制细胞(MDSC)在接种后睾丸中稳步下降。总的F4/80-CD11c+树突状细胞(DCs)维持相对稳定的水平,而常规1型树突状细胞(cDC1)从第1天到第28天逐渐增加。在第1天和第7天存在较低数量的CD4+和CD8+T细胞,并且它们在第28天具有相似的结果,具有上限水平。睾丸显示较高水平的CD3+T细胞,但巨噬细胞的频率较低,与附睾相比,cDC2和接种后28天的嗜中性粒细胞。总之,我们的数据表明急性附睾-睾丸炎可能导致睾丸的长期损伤,其特征在于CD3+T细胞(包括CD4+和CD8+T细胞)介导的免疫应答。
The testicular consequences of acute epididymo-orchitis remain largely unelucidated in long-term damage, which might be a neglected factor for male infertility. In this study, the differential phenotype of testicular immune cell subpopulations in lipopolysaccharide (LPS)-induced mouse epididymo-orchitis were analyzed by flow cytometry on day 1, day 7, and day 28. The number of macrophages, neutrophils, and myeloid-derived suppressor cells (MDSCs) steadily decreased in the testes with inoculation. Total F4/80-CD11c+ dendritic cells (DCs) maintained a relatively stable level, whereas conventional type 1 dendritic cells (cDC1) increased gradually from day 1 to day 28. There was a lower number of CD4+ and CD8+ T cells at day 1 and day 7, and they had similar results with a ceiling level at day 28. The testes displayed a higher level of CD3+ T cells but a lower frequency of macrophages, cDC2, and neutrophils at 28 days post-inoculation compared with the epididymis. In summary, our data indicates acute epididymo-orchitis could lead to long-term damage in the testes, which is characterized by CD3+ T cell (including CD4+ and CD8+ T cells)-mediated immune responses.