西利克长度（SL）是重要的产量性状，与每个西利克种子和种子重量呈正相关。在本研究中,两个双单倍体（DH）种群，从杂交中双11×R11(ZR)和R1×R2(RR)建立，包含280和95条DH线，分别,用于绘制SL的数量性状位点（QTL）。构建了ZR种群的高密度遗传图谱，包括19个连锁群上的14,658个bin，图长为2,198.85cM，平均标记距离为0.15cM。通过使用锚定在19条染色体上的2,046个定位标记，具有2,217-cM的图谱长度和1.08cM的平均标记距离，构建了RR种群的遗传连锁图。从ZR和RR种群中鉴定出A09上的主要QTLqSL_ZR_A09和qSL_RR_A09b，分别。两种QTL均可在四种环境中稳定检测。QTLqSL_RR_A09b和qSL_ZR_A09位于68.5-70.8cM和91.33-91.94cM区间，RR和ZR种群的R2值为14.99-39.07%和15.00-20.36%，分别。基于拟南芥中qSL_ZR_A09侧翼的单核苷酸多态性(SNP)标记的物理位置和基因注释,鉴定了26个在亲本之间具有SNP/Indel变异的基因,并且选择两个基因(BnaA09g41180D和BnaA09g41380D)作为候选基因。表达分析进一步显示BnaA09g41180D，编码拟南芥fasciclin样阿拉伯半乳聚糖蛋白（FLA3）的同源物，作为qSL_ZR_A09最有希望的候选基因。QTL鉴定和候选基因分析将为控制甘蓝型油菜SL的基因组区域以及QTL基础的候选基因提供新的见解。
Silique length (SL) is an important yield trait and positively correlates with seeds per silique and seed weight. In the present study, two double haploid (DH) populations, established from crosses Zhongshuang11 × R11 (ZR) and R1 × R2 (RR), containing 280 and 95 DH lines, respectively, were used to map quantitative trait loci (QTL) for SL. A high-dense genetic map from ZR population was constructed comprising 14,658 bins on 19 linkage groups, with map length of 2,198.85 cM and an average marker distance of 0.15 cM. Genetic linkage map from RR population was constructed by using 2,046 mapped markers anchored to 19 chromosomes with 2,217-cM map length and an average marker distance of 1.08 cM. Major QTL qSL_ZR_A09 and qSL_RR_A09b on A09 were identified from ZR and RR populations, respectively. Both QTL could be stably detected in four environments. QTL qSL_RR_A09b and qSL_ZR_A09 were located on 68.5-70.8 cM and 91.33-91.94 cM interval with R2 values of 14.99-39.07% and 15.00-20.36% in RR and ZR populations, respectively. Based on the physical positions of single nucleotide polymorphism (SNP) markers flanking qSL_ZR_A09 and gene annotation in Arabidopsis, 26 genes were identified with SNP/Indel variation between parents and two genes (BnaA09g41180D and BnaA09g41380D) were selected as the candidate genes. Expression analysis further revealed BnaA09g41180D, encoding homologs of Arabidopsis fasciclin-like arabinogalactan proteins (FLA3), as the most promising candidate gene for qSL_ZR_A09. The QTL identification and candidate gene analysis will provide new insight into the genomic regions controlling SL in Brassica napus as well as candidate genes underlying the QTL.
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