口腔扁平苔藓（OLP）和口腔苔藓样病变（OLL）是涉及口腔粘膜的慢性炎症性疾病。OLP和OLL中的B细胞浸润，然而,对OLP和OLL中的这些细胞知之甚少。分析OLP和OLL中B淋巴细胞的功能和浸润特征。并初步评估其与临床结局的相关性。从OLP收集组织样本，OLL,和健康的粘膜。通过单细胞测序分析组织中B细胞的表型和数量。通过免疫组织化学和免疫荧光检查它们在组织中的比例和浸润特征。随着皮质激素的全身用药，评估了B细胞浸润特征与临床结局之间的相关性.在OLP和OLL中均显示出B细胞的定量比例增加。OLP中的B细胞在免疫应答中表现出增强的激活和增强的调节。检查了100名OLP/OLL患者和13名健康对照的队列，以研究B细胞浸润模式。OLP和OLL的B细胞分布在固有层表层的92.9%和41.9%，分别(P<0.01)；OLP和OLL的局部分布为25.0%和62.9%，分别为(P<0.01)。随着皮质激素的全身用药，OLP组和OLL组有B细胞浸润(B+)的患者治疗前后REU评分降低有统计学意义(P<0.01)。B细胞广泛存在于OLP和OLL中，OLP和OLL中的B细胞浸润与口服皮质激素更好的治疗效果有关。
Oral lichen planus (OLP) and oral lichenoid lesion (OLL) are chronic inflammatory diseases involving the oral mucosa. B cells infiltration in OLP and OLL, however, little is known about these cells in OLP and OLL. To analyze the function and infiltrating features of B lymphocytes in OLP and OLL, and to preliminarily evaluate their correlation with clinical outcomes. Tissue samples were collected from OLP, OLL, and healthy mucosa. The phenotypes and amounts of B cells in tissues were analyzed by single-cell sequencing. Their proportion and infiltrating features in tissues were examined by immunohistochemistry and immunofluorescence. With the systemic medication of
corticoids, the correlation between B cells infiltrating characteristics and the clinical outcomes were evaluated. A quantified proportion increase of B cells was shown in both OLP and OLL. B cells in OLP demonstrated heightened activation and enhanced regulation in immune response. A cohort of 100 patients with OLP/OLL and 13 healthy controls were examined to investigate the B cells infiltration pattern. B cells were distributed in the superficial layer of lamina propria in 92.9% and 41.9% of OLP and OLL, respectively(P < 0.01); focally distributed in 25.0% and 62.9% of OLP and OLL, respectively(P < 0.01). With the systemic medication of
corticoids, the cases with B cell infiltration (B+) in OLP and OLL groups showed a statistically significant reduction in REU scores before and after treatment (P < 0.01). B cells are widely present in OLP and OLL, and B cell infiltration in OLP and OLL are related to the better therapeutic effect of oral
corticoids.
