利用有益的微生物,即,益生菌,减少病原体并提高目标物种的性能是海水养殖的重要管理策略。本研究旨在探讨四种微生物的潜力,汉森德巴酵母,PollisRuegeria,植物乳杆菌,和枯草芽孢杆菌,抑制弧菌和增加存活率,种群增长和消化酶活性(蛋白酶,脂肪酶,和淀粉酶)在类食足类co足类中,刺槐。Co足类动物,将初始平均密度为50个个体/mL的刺槐原种培养物(25个成年雄性和25个成年雌性)分配到五个处理中(即,四个实验和一个对照,每个重复四次;重复两次)使用20个烧杯(每个100mL容量)。co足类被饲喂鞭毛藻Alexandriumtamarense和硅藻Phyaeodactylumtricornutum的混合物(3×104个细胞/mL-1)。将每种微生物的浓度调节至108CFU/mL-1并应用于培养条件。D.Hansenii,植物乳杆菌,和枯草芽孢杆菌都改善了co足类动物的生存和种群增长,可能包括较高的脂肪酶活性(P<0.05)。相比之下,与对照组相比,使用动动R.没有改善co足动物的培养性能。枯草芽孢杆菌对减少co足类的外部和内部弧菌负荷最有效。在饥饿期间,co足类中的益生菌浓度在几天内下降,这表明需要常规重新应用益生菌来维持微生物种群及其提供的益处。我们的结果表明,D.hansenii和B.subtilis是有希望的益生菌,可用于大量co足动物培养,作为海水养殖目的的活食。
The use of beneficial microbes, i.e., probiotics, to reduce pathogens and promote the performance of the target species is an important management strategy in mariculture. This study aimed to investigate the potential of four microbes, Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum, and Bacillus subtilis, to suppress Vibrio and increase survival, population growth and digestive enzyme activity (protease, lipase, and amylase) in the harpacticoid
copepod, Tigriopus japonicus.
Copepod, T. japonicus stock culture with an initial mean density of 50 individual/mL (25 adult male and 25 adult female) was distributed into five treatments (i.e., four experimental and a control, each with four replicates; repeated twice) using 20 beakers (100 mL capacity each). The copepods were fed a mixture of the dinoflagellate Alexandrium tamarense and the diatom Phyaeodactylum tricornutum (3 × 104 cells/mL-1). Each microbe\'s concentration was adjusted at 108 CFU/mL-1 and applied to the culture condition. D. hansenii, L. plantarum, and B. subtilis all improved the copepods\' survival and population growth, likely by including a higher lipase activity (P < 0.05). In contrast, using R. mobilis did not improve the
copepod\'s culture performance compared to control. B. subtilis was the most effective in decreasing the
copepod\'s external and internal Vibrio loading. The probiotic concentrations in the
copepod decreased within days during starvation, suggesting that routine re-application of the probiotics would be needed to sustain the microbial populations and the benefits they provide. Our results demonstrated that D. hansenii and B. subtilis are promising probiotics for mass copepod culture as live food for mariculture purposes.