目的:目的是评估LTF之间的关联,MMP20、CA6和TAS1R2基因多态性与壮族人群龋齿易感性的关系及探讨乳铁蛋白对龋齿易感性影响的潜在机制。
方法:对广西315名青少年进行了病例对照研究,中国,2022年5月至11月。通过口试和问卷调查收集数据。使用SNPscan技术收集颊粘膜细胞和DNA样品。从69名具有各种LTFrs10865941基因型的受试者中采集唾液和牙龈上斑块样品。LTFrs10865941多态性之间的关系,乳铁蛋白,变形链球菌,用ELISA和qRT-PCR检测龋齿,以及Logistic回归分析。
结果:LTF基因的基因型分布在病例组和对照组之间存在显着差异(p=0.018)。病例组比对照组具有更低的C等位基因和更高的T等位基因频率(p=0.006)。LTFrs10865941多态性与显性龋齿有关,支配,和加法模型(p<0.05)。MMP20rs1784418、CA6rs2274328、TAS1R2rs35874116两组间无明显差别(p>0.05)。更多的变形链球菌。在病例组中发现了牙龈上斑块(p=0.03)。两组在共显性模型和显性模型中均存在显着差异(p<0.05)。
结论:在中国壮族人群中,LTFrs10865941多态性可能与龋齿易感性有关。LTFrs10865941T等位基因可能是龋齿的潜在危险因素。
OBJECTIVE: The aim was to assess the associations between the LTF, MMP20, CA6, and TAS1R2 polymorphisms and caries in the Zhuang population and explore the underlying mechanism of the impact of lactoferrin on caries susceptibility.
METHODS: A case-control study of 315 adolescents was conducted in Guangxi,
China, from May-November 2022. Data were collected through oral examinations and questionnaires. Buccal mucosa cells and DNA samples were collected using the SNPscan technique. Saliva and supragingival plaque samples were taken from 69 subjects with various LTF rs10865941 genotypes. The relationships among the LTF rs10865941 polymorphism, lactoferrin, Streptococcus mutans, and caries were investigated by using the ELISA and qRT-PCR, along with logistic regression analysis.
RESULTS: The genotype distribution of the LTF gene were significantly different between the case and control groups (p = 0.018). The case group had lower C allele and greater T allele frequencies than the control group (p = 0.006). The LTF rs10865941 polymorphism was associated with caries in the codominant, dominant, and additive models (p < 0.05). MMP20 rs1784418, CA6 rs2274328, and TAS1R2 rs35874116 were not significantly different between the two groups (p > 0.05). A greater quantity of S. mutans. in the supragingival plaque was found in the case group (p = 0.03). There were significant differences between the two groups in both the codominant model and the dominant model (p < 0.05).
CONCLUSIONS: The LTF rs10865941 polymorphism may be associated with caries susceptibility in the Zhuang population of
China. The LTF rs10865941 T allele may be a potential risk factor for dental caries.