生物样品中目标物质的含量通常处于低浓度水平,生物样品的基质通常很复杂。样品制备被认为是生物分析中非常关键的步骤。目前,在生物分析领域,微萃取采样技术的应用已相当普遍。目前,该领域的关键发展集中在高效的微萃取介质以及可适应的样品制备方法的小型化和自动化上。在这次审查中,从微萃取介质的制备和微萃取采样策略的角度介绍了用于生物分析的微萃取采样技术的最新进展。详细综述了微萃取介质的研究进展,主要包括适体功能化材料,分子印迹聚合物,碳基材料,金属有机框架,共价有机骨架,等。总结了微量提取采样技术的进展,主要是基于体内采样,体外采样和微透析技术。此外,简要讨论了生物分析用微量萃取取样技术的当前挑战和未来趋势。
The contents of target substances in biological samples are usually at low concentration levels, and the matrix of biological samples is usually complex. Sample preparation is considered a very critical step in
bioanalysis. At present, the utilization of microextraction sampling technology has gained considerable prevalence in the realm of biological analysis. The key developments in this field focus on the efficient microextraction media and the miniaturization and automation of adaptable sample preparation methods currently. In this review, the recent progress on the microextraction sampling technologies for
bioanalysis has been introduced from point of view of the preparation of microextraction media and the microextraction sampling strategies. The advance on the microextraction media was reviewed in detail, mainly including the aptamer-functionalized materials, molecularly imprinted polymers, carbon-based materials, metal-organic frameworks, covalent organic frameworks, etc. The advance on the microextraction sampling technologies was summarized mainly based on in-vivo sampling, in-vitro sampling and microdialysis technologies. Moreover, the current challenges and perspective on the future trends of microextraction sampling technologies for
bioanalysis were briefly discussed.