■华支睾吸虫病是一种重要的食源性寄生虫病。然而,华支睾吸虫卵(C.sinensis)在胆道梗阻期间无法在粪便中检测到。此外,很多疾病都会导致胆道梗阻,比如胆结石,腺癌,胆管癌和蛔虫感染。因此,对鉴别胆道梗阻患者和合并中华绒螯蟹感染的胆道梗阻患者具有重要意义。
■共纳入48例胆道梗阻患者,包括23例感染了C.sinensis(C.sinensis)(OB+C.s)和25个未感染的受试者(OB)。通过内镜逆行胰胆管造影术收集胆汁样品,并使用超高效液相色谱-四极杆飞行时间质谱(UHPLC-QTOFMS)进行分析。此外,采用多变量统计分析方法鉴定差异代谢物。接下来,通过靶向代谢组学分析确定胆汁氨基酸水平.
■在电喷雾电离(ESI)和ESI-模式下,总共鉴定出146和132种重要的代谢物,分别。氨基酸(天冬酰胺,谷氨酸,鸟氨酸)和多胺(亚精胺和精胺)发生了显着变化。有针对性的分析表明,氨基酸(如L-精氨酸,L-谷氨酰胺,L-赖氨酸,L-丙酸,和L-酪氨酸)在OB+C中较低与OB患者相比。标记的代谢途径参与“谷胱甘肽代谢”,“咖啡因代谢”,\"丙氨酸,天冬氨酸和谷氨酸代谢“精氨酸和脯氨酸代谢”,“嘌呤代谢”,“β-丙氨酸代谢”,和“D-谷氨酰胺和D-谷氨酸代谢”。
■这些结果表明OB+C之间存在显著差异s和OB患者,尤其是氨基酸。代谢特征和代谢途径中的扰动可能有助于更好地区分OB+C。s和OB患者。
Clonorchiasis is an important foodborne parasitic disease. However, eggs of Clonorchis sinensis (C. sinensis) cannot be detected in feces during biliary obstruction. Moreover, many diseases can cause biliary obstruction, such as gallstones, adenocarcinoma, cholangiocarcinoma and Ascaris lumbricoides infection. Therefore, it is of great significance to distinguish between patients of biliary obstruction and biliary obstruction with C. sinensis infection.
A total of 48 biliary obstruction patients were enrolled, including 23 infected with C. sinensis (C. sinensis) (OB+C.s) and 25 non-infected subjects (OB). The bile samples were collected by endoscopic retrograde cholangiopancreatography and analyzed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF MS). Additionally, multivariate statistical analysis methods were employed to identify differential metabolites. Next, bile amino acid levels were determined by targeted metabolomics analysis.
A total of 146 and 132 significant metabolites were identified in electrospray ionization (ESI)+ and ESI- modes, respectively. The levels of amino acids (asparagine, glutamate, ornithine) and polyamines (spermidine and spermine) were significantly changed. Targeted analysis showed that the levels of amino acids (such as L-arginine, L-glutamine, L-lysine, L-propionic, and L-tyrosine) were lower in OB+C.s patients compared to those in OB patients. Marked metabolic pathways were involved in \"Glutathione metabolism\", \"Caffeine metabolism\", \"Alanine, aspartate and glutamate metabolism\", \"Arginine and proline metabolism\", \"Purine metabolism\", \"Beta-Alanine metabolism\", and \"D-glutamine and D-glutamate metabolism\".
These results show that there were significant differences between OB+C.s and OB patients, especially in amino acids. The metabolic signature and perturbations in metabolic pathways may help to better distinguish OB+C.s and OB patients.