尽管全球努力管理水体富营养化,沉积物中氨氮的不断释放维持了水体的富营养化状态,给管理层带来了严峻的挑战。为了找到一种有效的泥沙修复方法,进行了利用信号分子增强微生物在沸石上粘附的实验。使用两种不同的信号分子检查了五种不同的沸石铵吸附,N-(3-氧代己酰基)-L-高丝氨酸内酯(OHHL)和N-(β-酮己酰基)-DL-高丝氨酸内酯(C6),以增强微生物在两种类型的沸石上的附着。结果表明,用信号分子C6增强的改性微生物附着Z1沸石效果最好。在高氨吸附的情况下效果更好,TN去除率可达7.99mg·L-1,抑制率为90.08%。氨氮去除率达到4.75mg·L-1,抑制率为87.64%,上覆水的氨氮和总氮达到地表Ⅲ水质标准。此外,信号分子的加入增加了细菌胶体表面的ζ电位。此外,溶解有机物(DOM)部分中蛋白质I的含量增加,提高微生物粘附能力,促进其附着在沸石表面。信号分子C6可以增加微生物表面的ζ电位,促进蛋白质I的产生,从而加强沸石生物膜的附着,改善水质。
Despite global efforts to manage water eutrophication, the continual release of ammonia nitrogen from sediments maintains the eutrophic state of water bodies, presenting serious challenges to the management. In order to find an efficient method for sediment remediation, the experiment of using signal molecules to enhance the adhesion of microorganisms on zeolite was carried out. Five different zeolitic ammonium adsorptions were examined using two different signal molecules, N-(3-oxohexanoyl)-L-homoserine lactone (OHHL) and N-(β-ketocaproyl)-DL-homoserine lactone (C6), to enhance microbial attachment on two types of zeolites. The results showed that the modified microbial attached Z1 zeolite reinforced with signal molecule C6 had the best effect. The effect was better in the case of high ammonium adsorption, and the TN removal could reach 7.99 mg·L-1 with an inhibition rate of 90.08%. The ammonia nitrogen removal reached 4.75 mg·L-1 with an inhibition rate of 87.64%, and the ammonia nitrogen and total nitrogen of the overlying water reached the surface III water quality standard. In addition, the addition of the signal molecule increased the zeta potential on the surface of the bacterial colloid. In addition, the amount of protein I in the dissolved organic matter (DOM) fraction increased, improving microbial adhesion ability and facilitating their attachment to the zeolite surface. The signal molecule C6 could increase the zeta potential of microbial surface and promote the production of protein I, thus strengthening the attachment of zeolite biofilm and improving the water quality.