■这项研究的目的是确定临床分布,烟曲霉的体外抗真菌药敏及抗性机制(A.烟曲霉)于2021年11月至2023年5月从宁夏医科大学总医院分离出。
■抗真菌药敏试验使用SensititreYeastOneYO10进行,并使用临床和实验室标准研究所(CLSI)M38-第三版建立的标准肉汤微量稀释测定法进一步确认具有高最低抑菌浓度(MIC)的分离株。在唑抗性的烟曲霉菌株中进行全基因组重测序和RT-qPCR以研究潜在的抗性机制。
■总的来说,从各个临床科室共鉴定出276株烟曲霉分离株,在过去3年中,分离株的数量呈增加趋势。观察到两株耐唑的烟曲霉(0.72%),其中一个显示过表达cyp51A,cyp51B,cdr1B,MDR1/2,artR,srba,erg24A,和er4B,但没有cyp51A突变.然而,另一种菌株在cyp51A序列(L98H/S297T)中具有两个改变。因此,我们首先描述了宁夏两株耐唑的临床烟曲霉,中国,并报道了一种唑抗性菌株,该菌株在cyp51A基因中具有L98H/S297T突变,在启动子区域中没有任何串联重复(TR)序列。
■本研究强调了加强对唑类耐药烟曲霉的关注和监测的重要性,特别是那些具有cyp51A或非cyp51A突变的非TR点突变的人,以便更好地了解它们在该地区的流行和传播。
UNASSIGNED: The objective of this study was to determine the clinical distribution, in vitro antifungal susceptibility and underlying resistance mechanisms of Aspergillus fumigatus (A. fumigatus) isolates from the General Hospital of Ningxia Medical University between November 2021 and May 2023.
UNASSIGNED: Antifungal susceptibility testing was performed using the Sensititre YeastOne YO10, and isolates with high minimal inhibitory concentrations (MICs) were further confirmed using the standard broth microdilution assays established by the Clinical and Laboratory Standards Institute (CLSI) M38-third edition. Whole-Genome Resequencing and RT-qPCR in azole-resistant A. fumigatus strains were performed to investigate the underlying resistance mechanisms.
UNASSIGNED: Overall, a total of 276 A. fumigatus isolates were identified from various clinical departments, showing an increasing trend in the number of isolates over the past 3 years. Two azole-resistant A. fumigatus strains (0.72%) were observed, one of which showed overexpression of cyp51A, cyp51B, cdr1B, MDR1/2, artR, srbA, erg24A, and erg4B, but no cyp51A mutation. However, the other strain harbored two alterations in the cyp51A sequences (L98H/S297T). Therefore, we first described two azole-resistant clinical A. fumigatus strains in Ningxia,
China, and reported one azole-resistant strain that has the L98H/S297T mutations in the cyp51A gene without any tandem repeat (TR) sequences in the promoter region.
UNASSIGNED: This study emphasizes the importance of enhancing attention and surveillance of azole-resistant A. fumigatus, particularly those with non-TR point mutations of cyp51A or non-cyp51A mutations, in order to gain a better understanding of their prevalence and spread in the region.