未经证实:据报道,哮喘和过敏性鼻炎与遗传因素密切相关。本研究的目的是评估TaqMan-MGB(小沟结合剂)qPCR方法检测CYSLTR1rs320995(T927C)和GSDMBrs7216389(G1199A)基因多态性的准确性,并探讨CYSLTR1rs320995和GSDMBrs7216389多态性与中国哮喘患者过敏性鼻炎易感性的相关性。
未经批准:在这项研究中,北京协和医院收治哮喘患者310例,健康人60例。通过TaqMan-MGBqPCR和DNA测序分析各组的CYSLTR1rs320995(T927C)和GSDMBrs7216389(G1199A)基因多态性。验证此方法后,选择71例过敏性鼻炎患者和72例哮喘合并过敏性鼻炎患者,并采用TaqMan-MGBqPCR进行检测.
UNASSIGNED:TaqMan-MGBqPCR结果与DNA测序结果完全一致(Kappa=1,P<0.001)。此外,TaqMan-MGBqPCR检测结果不受胆红素和血脂的影响.我们发现CYSLTR1rs320995基因型在女性哮喘合并过敏性鼻炎患者中的差异分布(χ2=6.172,P=0.046,统计学功效=0.591)。具体来说,TT基因型在患有过敏性鼻炎的哮喘女性中更为常见,而TC基因型在健康女性中更为普遍。然而,在GSDMBrs7216389多态性中未观察到这种关联.
UNASSIGNED:我们建立了可靠的TaqMan-MGBqPCR方法,用于检测CYSLTR1rs320995和GSDMBrs7216389多态性。此外,CYSLTR1rs320995多态性可能与中国女性哮喘和过敏性鼻炎患者的遗传易感性有关。未来需要具有更大样本量的多中心研究。
UNASSIGNED: Asthma and allergic rhinitis have been reported to be strongly associated with genetic factors. The aim of this study was to evaluate the accuracy of the TaqMan-MGB (minor groove binder) qPCR method for detecting CYSLTR1 rs320995 (T927C) and GSDMB rs7216389 (G1199A) gene polymorphisms as well as to explore the association of CYSLTR1 rs320995 and GSDMB rs7216389 polymorphisms with genetic susceptibility of Chinese patients with asthma and allergic rhinitis.
UNASSIGNED: In this study, 310 asthmatic patients and 60 healthy individuals were recruited in Peking Union Medical College Hospital. The CYSLTR1 rs320995 (T927C) and GSDMB rs7216389 (G1199A) gene polymorphisms in each group were analyzed by TaqMan-MGB qPCR and DNA sequencing which was regarded as the gold standard. After the validation of this method, additional 71 patients with allergic rhinitis and 72 patients with asthma combined with allergic rhinitis were selected and tested by using TaqMan-MGB qPCR.
UNASSIGNED: The TaqMan-MGB qPCR results were fully consistent with DNA sequencing results (Kappa = 1, P<0.001). In addition, the results of the TaqMan-MGB qPCR assay were not affected by bilirubin and lipids. We found differential distribution of CYSLTR1 rs320995 genotypes in female patients with asthma combined with allergic rhinitis (χ 2=6.172, P=0.046, statistical power = 0.591). Specifically, the TT genotype is more frequent in women suffering from asthma with allergic rhinitis, whereas the TC genotype is more prevalent in healthy women. However, no such associations were observed in the GSDMB rs7216389 polymorphism.
UNASSIGNED: We have established a reliable TaqMan-MGB qPCR method for the detection of CYSLTR1 rs320995 and GSDMB rs7216389 polymorphisms. Moreover, the CYSLTR1 rs320995 polymorphism may be associated with genetic susceptibility of Chinese female patients with asthma and allergic rhinitis. Multicenter studies with larger sample sizes are required in the future.