目的:本研究通过研究橡胶籽油(RSO)对氧化型低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮细胞(HUVECs)损伤的保护作用及其机制,来表征橡胶籽油(RSO)对动脉粥样硬化(AS)的抗氧化和抗炎作用。
方法:HUVECs采用RSO治疗,ox-LDL,RSO+ox-LDL,分别,接下来是细胞活性测试,IL-1β水平,IL-6,IL-10,TNF-α,ROS,NO,eNOSmRNA表达和MCP-1、VCAM-1、eNOS蛋白表达,TLR4、NF-κBp65、p-NF-κBp65。
结果:与ox-LDL组相比,细胞活力,NO程度和eNOSmRNA表达显著增高。以及IL-1β等促炎因子的水平,IL-6,TNF-α,IL-10、ROS显著降低,伴随着TLR4mRNA的减少,TLR4、MCP-1、VCAM-1蛋白表达,以及用250μg/mlox-LDL+50μg/mlRSO治疗组的NF-κBp-p65/p65比值,250μg/mlox-LDL+100μg/mlRSO,250μg/mlox-LDL+150μg/mlRSO。
结论:RSO可以降低促炎介质的表达,氧化因子参与血管内皮细胞损伤,表现出抗炎和抗氧化特性的HUVECs暴露于ox-LDL。此外,其可能通过抑制TLR4/NF-κB信号通路减轻内皮细胞损伤。
OBJECTIVE: This study was conducted to characterize the antioxidant and anti-inflammatory properties of Rubber Seed Oil (RSO) against atherosclerosis (AS) through the study of the protective effects and mechanisms on human umbilical vein endothelial cells (HUVECs) injury induced by oxidized low-density lipoprotein (ox-LDL).
METHODS: HUVECs were treated with RSO, ox-LDL, RSO + ox-LDL, respectively, followed by cell activity testing, levels of IL-1β, IL-6, IL-10, TNF-α, ROS, NO, the mRNA expression of eNOS and protein expression of MCP-1, VCAM-1, eNOS, TLR4, NF-κB p65、p-NF-κB p65.
RESULTS: Compared with the ox-LDL group, cell viability, NO level and the expression of eNOS mRNA significantly increased. and the levels of pro-inflammatory factors such as IL-1β, IL-6, TNF-α, IL-10, ROS were significantly decreased, which was accompanied by decreases in TLR4 mRNA, TLR4, MCP-1, VCAM-1 protein expression, as well as the ratio of NF-κB p-p65/p65 in the group treated with 250 μg/ml ox-LDL + 50 μg/ml RSO, 250 μg/ml ox-LDL + 100 μg/ml RSO, 250 μg/ml ox-LDL + 150 μg/ml RSO.
CONCLUSIONS: RSO can reduce the expression of pro-inflammatory mediators, oxidative factors involved in injured vascular endothelial cells, exhibiting anti-inflammatory and antioxidant properties HUVECs exposed to ox-LDL. In addition, it may alleviate endothelial cell damage by inhibiting the TLR4/NF-κB signaling pathway.