背景:本研究的目的是通过植入可流动树脂,在新的兔输尿管结石模型中描述输尿管结石阻塞引起的肾积水的病理生理变化。
方法:40只新西兰大白兔随机分为2组:结石组和假对照组。在结石组(n=20)中,兔子在左下腹部手术,左输尿管暴露。然后使用0.45mm直径的静脉内输注针将可流动树脂(可流动的修复牙科材料)注射到左输尿管中。然后通过牙科固化光光固化40s以形成结石。在假对照组中,将生理盐水注入输尿管。兔在术前接受X线和血常规及尿常规检查,还有X光片,CT,解剖,术后1、3、5、7天进行HE染色及血常规、尿常规检查。手术后通过X射线和未增强的CT扫描评估结石形成。通过解剖评估病理生理变化,HE染色及血常规、尿常规。
结果:17只兔成功构建输尿管结石模型。在微积分组中,术后第1天左侧下腹部可见高密度阴影,3rd,第5和第7位通过X射线和CT扫描。解剖发现左输尿管阻塞形成,手术后7天肾盂和输尿管上段扩张。左输尿管的肾脏长径仅在术后第一天增加。HE染色发现术后输尿管和肾脏损害。结石组和假手术组,血清肌酐,尿素氮,手术后第1天白细胞和尿红细胞升高.然而,指标在第3、5和7天恢复正常。
结论:这是一个稳定的,通过植入可流动树脂,减少了操作的复杂性和成本效益的输尿管结石模型。这种新的模型可能使我们进一步了解输尿管结石梗阻引起的病理生理变化。
BACKGROUND: The purpose of this study was to characterize the pathophysiological changes of hydronephrosis caused by ureteral calculi obstruction in a new rabbit ureteral calculi model by implanting flowable resin.
METHODS: Forty New Zealand rabbits were randomly divided into two groups: the calculi group and the sham control group. In the calculi group (n = 20), rabbits were operated at left lower abdomen and the left ureter was exposed. Then flowable resin (flowable restorative dental materials) was injected into the left ureter using a 0.45 mm diameter intravenous infusion needle. Then light-cured for 40 s by means of a dental curing light to form calculi. In the sham control group, normal saline was injected into the ureter. Rabbits underwent X-ray and routine blood and urine tests preoperatively, as well as X-ray, CT, dissection, HE staining and routine blood and urine tests on 1, 3, 5 and 7 days postoperatively. Stone formation was assessed by X-ray and unenhanced CT scan after surgery. The pathophysiological changes were evaluated through dissection, HE staining and routine blood and urine tests.
RESULTS: Ureteral calculi models were successfully constructed in 17 rabbits. In calculi group, high-density shadows were observed in the left lower abdomen on postoperative day 1st, 3rd, 5th and 7th by X-ray and CT scan. Dissection found obstruction formation of the left ureters, dilatation of the renal pelvis and upper ureter during 7 days after surgery. The renal long-diameters of the left ureters increased only on the 1st postoperative day. HE staining found ureteral and kidney damage after surgery. In calculi group and sham group,the serum creatinine, urea nitrogen, white blood cells and urine red blood cells were raised at day 1 after surgery. However, the indicators returned to normal at day 3, 5, and 7.
CONCLUSIONS: This is a stable, less complicated operation and cost-effective ureteral calculi model by implanting flowable resin. And this novel model may allow us to further understand the pathophysiology changes caused by ureteral calculi obstruction.