在这项研究中,我们使用16S高通量测序来研究铀矿开采对三种当地植物根际细菌群落和功能的影响,即,冷蒿,乌龙tatarionwiiSchott.,和SalixoritrephaSchneid.结果表明,铀矿开采显著降低了当地3种植物根际细菌的多样性,包括Shannon指数和Simpson指数(P<0.05)。有趣的是,我们发现,在铀矿区的三种当地植物的根际土壤中富集了鞘氨醇单胞菌和假杆菌,表明其重要的生态作用。这三种植物在铀矿区富集了各种优势根际细菌种群,包括Vicinamidobacteriaceae,诺卡诺德,和盖埃拉,这可能与植物根际独特的微生态环境有关。来自尾矿和露天矿的塔塔龙植物根际细菌群落也表现出一定程度的分化,表明铀矿开采是驱动高原植物根际土壤群落分化的主要因素。功能预测表明,来自不同植物的根际细菌具有不同的功能,以应对铀矿开采活动引起的胁迫。包括增强翻译拮抗剂Rof,翻译起始因子2B亚基,等。本研究首次探讨了高原铀矿开采活动对当地植物根际微生态的影响,推动建立有效的土壤微生态健康监测指标,为进一步修复高原铀矿区土壤污染提供参考。
In this study, we used 16S high-throughput sequencing to investigate the effects of
uranium mining on the rhizospheric bacterial communities and functions of three local plant species, namely, Artemisia frigida, Acorus tatarionwii Schott., and Salix oritrepha Schneid. The results showed that
uranium mining significantly reduced the diversity of rhizospheric bacteria in the three local plant species, including the Shannon index and Simpson index (P < 0.05). Interestingly, we found that Sphingomonas and Pseudotrichobacter were enriched in the rhizosphere soil of the three local plants from uranium mining areas, indicating their important ecological role. The three plants were enriched in various dominant rhizospheric bacterial populations in the
uranium mining area, including Vicinamidobacteriaceae, Nocardioides, and Gaiella, which may be related to the unique microecological environment of the plant rhizosphere. The rhizospheric bacterial community of A. tatarionwii plants from tailings and open-pit mines also showed a certain degree of differentiation, indicating that uranium mining is the main factor driving the differentiation of plant rhizosphere soil communities on the plateau. Functional prediction revealed that rhizospheric bacteria from different plants have developed different functions to cope with stress caused by uranium mining activities, including enhancing the translational antagonist Rof, the translation initiation factor 2B subunit, etc. This study explores for the first time the impact of plateau
uranium mining activities on the rhizosphere microecology of local plants, promoting the establishment of effective soil microecological health monitoring indicators, and providing a reference for further soil pollution remediation in plateau
uranium mining areas.