目的:通过对Ⅰ型胶原(ColⅠ)和Ⅲ型胶原(ColⅢ)的定量分析,研究强脉冲光(IPL)和烧蚀2.940nm掺铒钇铝石榴石(Er:YAG)激光对胶原动态变化的影响,转化生长因子(TGF),和兔真皮中的基质金属蛋白酶(MMPs)。
方法:将10只家兔的背部分为四个治疗区:IPL正常能量组,IPL高能组,铒激光正常能量组,和铒激光高能组。在第一次治疗后立即和第一次治疗后两周进行HE染色。上校I,ColIII,在基线时通过实时PCR收集TGF-β1和MMP-1,每次会议后2周,整个治疗后三个月每月一次。
结果:HE染色显示,四个治疗组真皮浅层胶原纤维均有不同程度的增加和增厚。Real-timePCR显示四组治疗前后各检查点差异有统计学意义,而4个治疗组的4种生物标志物变化无显著差异.
结论:IPL和Er:YAG激光均能有效上调胶原,但在这个实验中,四个照射组之间的治疗效果没有显着差异。此外,高能量组的表皮损伤更为严重。
OBJECTIVE: To investigate the effects of intense pulsed light (IPL) and ablative 2.940 nm erbium-doped yttrium aluminum garnet (Er: YAG) laser on dynamic changes in collagen by quantitative analysis of type I collagen (Col I) and type III collagen (Col III), transforming growth factor (TGF), and matrix metalloproteinases (MMPs) in the dermis of rabbits.
METHODS: Backs of ten rabbits were divided into four treatment areas: IPL normal energy group, IPL high-energy group, erbium laser normal energy group, and erbium laser high-energy group. HE staining was performed immediately after the first treatment and two weeks after the first treatment. Col I, Col III, TGF-β1, and MMP-1 were collected by real-time PCR at baseline, 2 weeks after each session, and three months after the entire treatment monthly.
RESULTS: HE staining showed that collagen fibers in the superficial layer of the dermis in the four treatment groups were increased and thickened to different degrees. Real-time PCR showed that statistically differences were noted in each checkpoint before and after treatments in the four groups, whereas no significant difference in the change in the four biomarkers was found among the four treatment groups.
CONCLUSIONS: Both the IPL and Er: YAG laser can effectively upregulate collagens, but in this experiment, there was no significant difference in the therapeutic effect among the four irradiation groups. Moreover, the high-energy level group tended to bring more serious epidermal injury.