本研究旨在探讨淫羊藿苷对精神分裂症的治疗作用及可能机制。SD大鼠分为5组,一个对照组,MK801诱发精神分裂症模型组,和三个淫羊藿苷治疗组,每组12只大鼠。采用Morris水迷宫和开放场观察大鼠的空间学习记忆能力。与对照组相比,MK801诱导模型组大鼠的刻板行为评分增加,自发活动的距离,逃逸延迟,丙二醛(MDA)含量,和IL-6,IL-1β,TNF-α表达,但平台杂交次数和超氧化物歧化酶(SOD)活性降低(P<0.05)。此外,淫羊藿苷治疗后模型组上述变化均呈剂量依赖性逆转(P<0.05)。网络药理学发现淫羊藿苷可以通过一些信号通路发挥抗精神分裂症作用,比如relaxin,雌激素,和TNF信号通路。MAPK1,MAPK3,FOS,RELA,TNF,JUN是淫羊藿苷治疗精神分裂症的关键目标,在动物模型中检测到它们的表达,这与网络药理学的预测结果一致。淫羊藿苷治疗可能通过TNF信号通路改善精神分裂症大鼠的空间学习记忆能力。
This study aims to explore the therapeutic effect and possible mechanisms of icariin in schizophrenia. SD rats were divided into five groups, a control group, a MK801-induced schizophrenia model group, and three icariin treatment groups, with twelve rats in each group. Morris water maze and open field were used to observe the spatial learning and memory ability of rats. Compared with the control group, rats in the MK801-induced model group showed an increase in stereotypic behavior score, distance of spontaneous activities, escape latency, malondialdehyde (MDA) content, and IL-6, IL-1β,
TNF-α expression, but a decrease in platform crossing times and superoxide dismutase (SOD) activity (P < 0.05). Furthermore, all the above changes of the model group were reversed after icariin treatment in a dose-dependent manner (P < 0.05). Network pharmacology found that icariin can exert anti-schizophrenic effects through some signaling pathways, such as relaxin, estrogen, and
TNF signaling pathways. MAPK1, MAPK3, FOS, RELA,
TNF, and JUN were the key targets of icariin on schizophrenia, and their expression was detected in animal models, which was consistent with the predicted results of network pharmacology. Icariin treatment may improve the spatial learning and memory ability of schizophrenic rats through
TNF signaling pathway.