背景:越来越多的研究表明环状RNA(circularRNAs,circRNAs)与癌症进展有关。CircRNAUBAP2与前列腺癌密切相关。然而,circUBAP2的生物学功能和具体机制在前列腺癌(PCa)中仍未被发现。
目的:本研究旨在探讨circUBAP2在PCa中的生物学功能和作用机制。
方法:通过qRT-PCR和Westernblot检测mRNA和蛋白水平,分别。细胞生长,迁移,使用CCK-8测定法和Transwell测定法测量侵袭能力。使用流式细胞术评估细胞凋亡。通过荧光素酶报告测定circUBAP2、miR-143和TFAP2B之间的相互作用。通过体内肿瘤形成测定来确定肿瘤生长。使用H&E染色测定评估肿瘤形态,并进行免疫组织化学测定以评估KI67的水平。
结果:我们发现circUBAP2和TFAP2B显著升高,而miR-143在前列腺癌细胞和组织中大幅减弱。CircUBAP2被发现影响细胞活力,转移和EMT,同时降低前列腺癌细胞的凋亡率。CircUBAP2直接靶向miR-143,miR-143抑制剂可以逆转circUBAP2干扰在前列腺癌细胞中诱导的作用。TFAP2B与miR-143直接结合,过表达TFAP2B可减轻miR-143在前列腺癌细胞中诱导的影响。
结论:CircUBAP2通过miR-143/TFAP2B轴促进前列腺癌进展。
BACKGROUND: More and more investigations reveal that circular RNAs (circRNAs) are involved in cancer progression. CircRNA UBAP2 was closely related to prostate cancer. However, the biological function and specifical mechanism of circUBAP2 are still poorly discovered in prostate cancer (PCa).
OBJECTIVE: This study aims to explore the biological function and mechanism of circUBAP2 in PCa.
METHODS: The levels of mRNA and proteins were assessed by qRT-PCR assay and Western blot, respectively. Cell growth, migration, and invasion ability were measured using CCK-8 assay and Transwell assay. Apoptosis was assessed using flow cytometry. The interactions between circUBAP2, miR-143, and
TFAP2B were determined by luciferase report assay. The tumor growth was determined by in vivo tumor formation assay. The tumor morphology was assessed using H&E staining assay, and immunohistochemistry assay was conducted to assess the level of KI67.
RESULTS: We found circUBAP2 and
TFAP2B were notably elevated, while miR-143 was largely attenuated in prostate cancer cells and tissues. CircUBAP2 was found to affect cell viability, metastasis and EMT, while attenuating the apoptosis rate of prostate cancer cells. CircUBAP2 directly targeted miR-143, and miR-143 inhibitor could reverse the effects that circUBAP2 interference-induced in prostate cancer cells.
TFAP2B is directly bound to miR-143, and overexpression of
TFAP2B could attenuate the influences that miR-143-induced in prostate cancer cells.
CONCLUSIONS: CircUBAP2 promoted prostate cancer progression via miR-143/
TFAP2B axis.